Czogalla, Jan and Schweda, Frank and Loffing, Johannes (2016) The Mouse Isolated Perfused Kidney Technique. JOVE-JOURNAL OF VISUALIZED EXPERIMENTS (117): e54712. ISSN 1940-087X,
Full text not available from this repository. (Request a copy)Abstract
The mouse isolated perfused kidney (MIPK) is a technique for keeping a mouse kidney under ex vivo conditions perfused and functional for 1 hr. This is a prerequisite for studying the physiology of the isolated organ and for many innovative applications that may be possible in the future, including perfusion decellularization for kidney bioengineering or the administration of anti-rejection or genome-editing drugs in high doses to prime the kidney for transplantation. During the time of the perfusion, the kidney can be manipulated, renal function can be assessed, and various pharmaceuticals administered. After the procedure, the kidney can be transplanted or processed for molecular biology, biochemical analysis, or microscopy. This paper describes the perfusate and the surgical technique needed for the ex vivo perfusion of mouse kidneys. Details of the perfusion apparatus are given and data are presented showing the viability of the kidney's preparation: renal blood flow, vascular resistance, and urine data as functional, transmission electron micrographs of different nephron segments as morphological readouts, and western blots of transport proteins of different nephron segments as molecular readout.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | RAT-KIDNEY; NORMOTHERMIC PERFUSION; EX-VIVO; TRANSPLANTATION; AUTOREGULATION; SECRETION; Physiology; Issue 117; isolated perfused kidney; mouse isolated perfused kidney; transplantation; organ perfusion; bioengineering; normothermic perfusion; renal physiology |
| Subjects: | 500 Science > 570 Life sciences |
| Divisions: | Biology, Preclinical Medicine > Institut für Physiologie > Prof. Dr. Frank Schweda |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 12 Apr 2019 11:59 |
| Last Modified: | 12 Apr 2019 11:59 |
| URI: | https://pred.uni-regensburg.de/id/eprint/3973 |
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