Vitzthum, Helga and Abt, Ilona and Einhellig, Stefan and Kurtz, Armin (2002) Gene expression of prostanoid forming enzymes along the rat nephron. KIDNEY INTERNATIONAL, 62 (5). pp. 1570-1581. ISSN 0085-2538, 1523-1755
Full text not available from this repository. (Request a copy)Abstract
Background. To obtain information about the general capability of nephron segments to elaborate prostanoids, we determined the gene expression of key enzymes for prostanoid formation. Methods. For this goal mRNAs were assayed for cyclooxygenases-1 and -2 as well as for the synthases of prostaglandin D-2 (PGD(2)), prostaglandin E-2 (PGE(2)), prostacyclin (PGI(2)) and thromboxane A(2) (TXA(2)) in microdissected rat nephron segments by RT-PCR. Results. Cyclooxygenase-1 (COX-1) mRNA was strongly expressed in all segments of the collecting ducts and to a lesser extent in glomeruli. COX-2 mRNA was found in the cortical thick ascending limb of Henle, and weaker expression also was detected in glomeruli. The lipocalin-type PGD synthase mRNA displayed a broad expression pattern in the cortex and outer medulla, including proximal convoluted tubule, thick ascending limb of Henle, distal convoluted tubule, and cortical and outer medullary collecting duct. The hematopoietic PGD synthase mRNA was restricted to the outer medullary collecting duct, and the membrane-associated PGE-synthase mRNA was exclusively expressed in the whole collecting duct system. Prostacylin-synthase mRNA was found in the whole kidney, but not in any microdissected nephron segment analyzed in this study. TXA-synthase mRNA was expressed in glomeruli. Conclusion. Given that the existence of cyclooxygenase in combination with the different PG-synthases is a prerequisite for the formation of prostanoids, our data suggest that PGD(2) is mainly formed in the thick ascending limb and in the collecting duct, while PGE(2) appears to be mainly generated by the collecting ducts. Probably no formation of PGI(2) occurs within the nephron. Whether TXA(2) can be formed by nephron segments remains questionable.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | PROSTAGLANDIN-F SYNTHASE; CYCLOOXYGENASE ISOFORMS; PROSTACYCLIN SYNTHASE; CELLULAR-LOCALIZATION; COLLECTING TUBULE; MESSENGER-RNA; E-2 SYNTHASE; MACULA DENSA; CDNA CLONING; KIDNEY; prostaglandin; thromboxane; cyclooxygenase; localization; nephron segments; blood flow; salt and water; renin secretion |
| Subjects: | 500 Science > 570 Life sciences |
| Divisions: | Biology, Preclinical Medicine > Institut für Physiologie > Prof. Dr. Armin Kurtz |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 04 Oct 2021 06:09 |
| Last Modified: | 04 Oct 2021 06:09 |
| URI: | https://pred.uni-regensburg.de/id/eprint/39740 |
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