Kellner, Karin and Liebsch, Gregor and Klimant, Ingo and Wolfbeis, Otto S. and Blunk, Torsten and Schulz, Michaela B. and Goepferich, Achim (2002) Determination of oxygen gradients in engineered tissue using a fluorescent sensor. BIOTECHNOLOGY AND BIOENGINEERING, 80 (1). pp. 73-83. ISSN 0006-3592
Full text not available from this repository.Abstract
Nutrient and oxygen supply of cells are crucial to tissue engineering in general. If a sufficient supply cannot be maintained, the development of the tissue will slow down or even fail completely. Previous studies on oxygen supply have focused on measurement of oxygen partial pressures (pO(2)) in culture media or described the use of invasive techniques with spatially limited resolution. The experimental setup described here allows for continuous, noninvasive, high-resolution pO(2) measurements over the cross-section of cultivated tissues. Applying a recently developed technique for time-resolved pO(2) sensing using optical sensor foils, containing luminescent O-2-sensitive indicator dyes, we were able to monitor and analyze gradients in the oxygen supply in a tissue over a 3-week culture period. Cylindrical tissue samples were immobilized on top of the sensors. By measuring the luminescence decay time, two-dimensional pO(2) distributions across the tissue section in contact with the foil surface were determined. We applied this technique to cartilage explants and to tissue-engineered cartilage. For both tissue types, changes were detected in monotonously decreasing gradients of pO(2) from the surface with high pO(2) to minimum pO(2) values in the center of the samples. Nearly anoxic conditions were observed in tissue constructs (similar to0 Torr) but not in excised cartilage discs (similar to20 Torr) after 1 day. Furthermore, the oxygen supply seemed to strongly depend on cell density and cell function. Additionally, histological analysis revealed a maximum depth of similar to1.3 mm of regular cartilage development in constructs grown under the applied culture conditions. Correlating analytical and histological analysis with the oxygen distributions, we found that pO(2) values below 11 Torr might impair proper tissue development in the center. The results illustrate that the method developed is an ideal one to precisely assess the oxygen demand of cartilage cultures. (C) 2002 Wiley Periodicals, Inc.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | ARTICULAR-CARTILAGE; POLYMER SCAFFOLDS; BIOREACTOR; OPTODES; CELLS; cartilage; oxygen; tissue engineering; optical oxygen sensor |
| Subjects: | 600 Technology > 600 Technology (Applied sciences) 600 Technology > 615 Pharmacy |
| Divisions: | Chemistry and Pharmacy > Institute of Pharmacy > Pharmaceutical Technology (Prof. Göpferich) Chemistry and Pharmacy > Institut für Analytische Chemie, Chemo- und Biosensorik |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 27 Aug 2021 06:00 |
| Last Modified: | 27 Aug 2021 06:00 |
| URI: | https://pred.uni-regensburg.de/id/eprint/39780 |
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