Kunz-Schughart, Leoni A. and Freyer, James P. (2002) Phosphorous metabolites and steady-state energetics of transformed fibroblasts during three-dimensional growth. AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY, 283 (4). C1287-C1297. ISSN 0363-6143
Full text not available from this repository. (Request a copy)Abstract
Rat1-T1 and MR1 spheroids represent separate transformed phenotypes originated from the same rat fibroblasts that differ in three-dimensional (3D) growth kinetics, histological structure, and oxygenation status. In the present study, P-31-NMR spectroscopy of perfused spheroid suspensions was used to investigate cellular energetics relative to 3D growth, development of necrosis, and cell cycle distribution. Both spheroid types were characterized by a remarkably low amount of free (inorganic) phosphate (Pi) and a low phosphocreatine peak. The ratio of nucleoside triphosphate (NTP) to P-i ranged between 1.5 and 2.0. Intracellular pH, NTP-to-P-i ratio, and NTP/cell remained constant throughout spheroid growth, being unaffected by the emergence of oxygen deficiency, cell quiescence, and necrosis. However, a 50% decrease in the ratio of the lipid precursors phosphorylcholine and phosphorylethanolamine (PC/PE) was observed with increasing spheroid size and was correlated with an increased G(1)/G(0) phase cell fraction. In addition, the ratio of the phospholipid degradation products glycerophosphorylcholine and glycerophosphorylethanolamine (GPC/GPE) increased with spheroid diameter in Rat1-T1 aggregates. We conclude that changes in phospholipid metabolism, rather than alterations in energy-rich phosphates, reflect cell quiescence in spheroid cultures, because cells in the inner oxygen-deficient zones seem to adapt their energy metabolism to the environmental conditions before necrotic cell destruction.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | MAGNETIC-RESONANCE SPECTROSCOPY; P-31 NMR-SPECTROSCOPY; MULTICELLULAR TUMOR SPHEROIDS; BREAST-CANCER CELLS; IN-VIVO; PHOSPHOLIPID-METABOLISM; MR SPECTROSCOPY; PHOSPHOGLYCERIDE METABOLISM; RADIOBIOLOGICAL HYPOXIA; MITOCHONDRIAL-FUNCTION; energy metabolism; tumor biology; nuclear magnetic resonance spectroscopy; phospholipids; quiescence |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Pathologie |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 04 Oct 2021 07:06 |
| Last Modified: | 04 Oct 2021 07:06 |
| URI: | https://pred.uni-regensburg.de/id/eprint/39794 |
Actions (login required)
 |
View Item |
