Reischl, Udo and Linde, Hans-Joerg and Lehn, Norbert and Landt, Olfert and Barratt, Kevin and Wellinghausen, Nele (2002) Direct detection and differentiation of Legionella spp. and Legionella pneumophila in clinical specimens by dual-color real-time PCR and melting curve analysis. JOURNAL OF CLINICAL MICROBIOLOGY, 40 (10). pp. 3814-3817. ISSN 0095-1137, 1098-660X
Full text not available from this repository. (Request a copy)Abstract
A dual-color LightCycler PCR assay targeting the 16S rDNA gene of Legionella spp. was established. By using two pairs of hybridization probes, Legionella spp. and Legionella pneumophila could be detected and differentiated simultaneously. With 26 culture-positive and 42 culture-negative respiratory specimens from patients with atypical pneumonia, 100% sensitivity and specificity was observed for L. pneumophila.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | POLYMERASE CHAIN-REACTION; BRONCHOALVEOLAR LAVAGE; RESPIRATORY SPECIMENS; LEGIONNAIRES-DISEASE; LIGHTCYCLER PCR; MIP GENE; IMMUNOASSAY; DIAGNOSIS; ANTIGEN; SAMPLES; |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Medizinische Mikrobiologie und Hygiene |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 05 Oct 2021 05:36 |
| Last Modified: | 05 Oct 2021 05:36 |
| URI: | https://pred.uni-regensburg.de/id/eprint/39833 |
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