Liss, Michael and Petersen, Birgit and Wolf, Hans and Prohaska, Elke (2002) An aptamer-based quartz crystal protein biosensor. ANALYTICAL CHEMISTRY, 74 (17). pp. 4488-4495. ISSN 0003-2700
Full text not available from this repository.Abstract
We developed a quartz crystal biosensor designed to detect concentrations and ligand afffinity parameters of free unlabeled proteins in real time. Using a model system with human IgE as the analyte and single-stranded DNA aptamers or an anti-IgE antibody as immobilized ligands, we could demonstrate that aptamers were equivalent to antibodies in terms of specificity and sensitivity. Both receptor types selectively detected 0.5 nmol/L of IgE. In addition, the aptamer receptors tolerated repeated affine layer regeneration after ligand binding and recycling of the biosensor with little loss of sensitivity. Because of the small size and nonprotein nature of the aptamers, they were immobilized in a dense, well-oriented manner, thus extending the linear detection range to 10-fold higher concentrations of IgE. In addition to demonstrating for the first time that an aptamer-based biosensor can specifically and quantitatively detect an analyte in various complex protein mixes, the aptamer-ligand proved to be relatively heat resistant and stable over several weeks. Since aptamers consist of nucleic acids, well-established chemistry can be applied to produce optimized affine layers on biosensors that may be developed to specifically detect proteins in solution for analysis of proteomes.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | HIGH-AFFINITY; SYSTEMATIC EVOLUTION; LIGANDS; RNA; BINDING; MICROBALANCE; RECOGNITION; MOLECULES; |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Medizinische Mikrobiologie und Hygiene |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 01 Sep 2021 10:26 |
| Last Modified: | 01 Sep 2021 10:26 |
| URI: | https://pred.uni-regensburg.de/id/eprint/39881 |
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