Schulz, Christian and Farkas, L and Wolf, K and Kratzel, K and Eissner, G and Pfeifer, M (2002) Differences in LPS-induced activation of bronchial epithelial cells (BEAS-2B) and type II-like pneumocytes (A-549). SCANDINAVIAN JOURNAL OF IMMUNOLOGY, 56 (3). pp. 294-302. ISSN 0300-9475
Full text not available from this repository. (Request a copy)Abstract
Lipopolysaccharide (LPS) as a major component of the outer membrane of gram-negative bacteria stimulates various cells to initiate a signalling cascade which ultimately leads to cell activation and expression of immunoregulatory or inflammatory cytokines. The human respiratory epithelium is an important environmental interface, but differences in LPS-induced cell activation between bronchial and alveolar epithelial cells have not yet been investigated in detail. First, the expression of Toll-like receptors (TLRs), as pattern-recognition receptors, was investigated for the bronchial epithelial cells and type II-like pneumocytes, demonstrating that they fulfil the prerequisites for LPS signalling. Thereafter, the effects of LPS, soluble CD14 (sCD14) and LPS-binding protein (LBP) on the release of interleukin-6 (IL-6) and IL-8 were studied. In the presence of LPS, sCD14 induced a significant and concentration-dependent cytokine release in type II-like pneumocytes, whereas the response of bronchial epithelial cells to sCD14 stimulation was low, implicating sCD14-independent activation mechanisms. Furthermore, LBP revealed inhibitory effects on the activation of alveolar epithelial cells, which may represent a novel local defence mechanism during gram-negative infection. We conclude that distinct pathways exist for LPS-induced activation of bronchial and alveolar epithelial cells.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | LIPOPOLYSACCHARIDE-BINDING-PROTEIN; NF-KAPPA-B; INVASIVE SHIGELLA-FLEXNERI; GRAM-NEGATIVE BACTERIA; SOLUBLE CD14; TOLL; EXPRESSION; ENDOTOXIN; SHOCK; MICE; |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Innere Medizin I Medicine > Lehrstuhl für Innere Medizin II |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 11 Oct 2021 08:16 |
| Last Modified: | 11 Oct 2021 08:16 |
| URI: | https://pred.uni-regensburg.de/id/eprint/39953 |
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