Staiger, Harald and Haering, Hans-Ulrich and Loeffler, Georg (2002) Serum-free differentiation of 3T3-L1 preadipocytes is characterized by only transient expression of peroxisome proliferator-activated receptor-gamma. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 296 (1). pp. 125-128. ISSN 0006-291X
Full text not available from this repository.Abstract
The adipogenic transcription factor peroxisome proliferator-activated receptor-gamma (PPARgamma) plays a pivotal role in the regulation of whole body insulin sensitivity. Little, however, is known about hormonal and molecular modulation of PPARgamma gene expression. Therefore, we investigated the temporal and conditional expression of PPARgamma in a serum-free model of 3T3-L1 adipocyte differentiation. We show here that expression of PPARgamma depends on the full set of known adipogenic stimuli and not on a single hormone/agent. Unexpectedly, an indeed marked but only transient peak of PPARgamma expression (39 +/- 5-fold increase over basal on day 3 after hormonal stimulation) occurs during serum-free adipose conversion. To our knowledge, this finding is novel and probably remained hidden until now because of the common use of serum-containing preadipocyte culture systems. We conclude that maintenance, but not induction, of PPARgamma gene expression in vitro must be achieved by one or more still unknown serum component(s). (C) 2002 Elsevier Science (USA). All rights reserved.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | GROWTH-FACTOR; PPAR-GAMMA; ADIPOGENESIS; ADIPOCYTES; CELLS; peroxisome proliferator-activated receptor-gamma; 3T3-L1; adipocyte differentiation; adipose conversion; platelet-derived growth factor; insulin sensitivity; insulin resistance |
| Subjects: | 500 Science > 540 Chemistry & allied sciences |
| Divisions: | Biology, Preclinical Medicine > Institut für Biochemie, Genetik und Mikrobiologie |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 07 Sep 2021 05:24 |
| Last Modified: | 07 Sep 2021 05:24 |
| URI: | https://pred.uni-regensburg.de/id/eprint/39984 |
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