Schreiber, Rainer and Kunzelmann, Karl (2016) Expression of anoctamins in retinal pigment epithelium (RPE). PFLUGERS ARCHIV-EUROPEAN JOURNAL OF PHYSIOLOGY, 468 (11-12). pp. 1921-1929. ISSN 0031-6768, 1432-2013
Full text not available from this repository. (Request a copy)Abstract
The anoctamin (ANO, TMEM16) family of Ca2+-activated Cl- channels consists of ten members with different cellular functions (ANO1-10). ANO1 is a Ca2+-activated Cl- channel in secretory epithelial cells of exocrine pancreas, salivary glands, or enterocytes. Expression of ANO1 also promotes cell proliferation and migration of tumor cells. ANO6 is essential for Ca2+-dependent scrambling of membrane phospholipids in platelets, red blood cells, and lymphocytes. ANO10 modulates Ca2+ signals in macrophages and has a role in cerebellar ataxia and other neurological disorders. All three anoctamins have been proposed to control intracellular Ca2+ signals. Anoctamins may also form the basolateral Ca2+-activated Cl- channel in the retinal pigment epithelium (RPE). We show that native human, bovine, porcine, and mouse RPEs express ANO1, ANO6, and ANO10. Growth arrested and confluent RPR cells expressed ANO1 in the plasma membrane, whereas ANO6 and ANO10 were found in the primary cilium. Ussing chamber experiments showed that the application of ATP to the apical (retinal) side of porcine RPE induced a Ca2+-activated Cl- secretion. Activation was inhibited by basolateral (choroidal) administration of the ANO inhibitors AO1, niflumic acid (NFA), and 4,4'-diisothiocyano-2,2'-stilbenedisulfonic acid (DIDS). The results suggest that ANO1 is responsible for basolateral Ca2+-dependent Cl-secretion in RPE, whereas ANO6 and ANO10 may have different functions, such as modulating Ca2+ signals.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | RECESSIVE CEREBELLAR-ATAXIA; CHLORIDE CHANNEL; VOLUME REGULATION; ANO10 MUTATIONS; CL-CHANNELS; CELLS; BESTROPHIN; TMEM16A; CA2+; CONTRIBUTES; TMEM16A; TMEM16F; TMEM16K; Anoctamin1; Anoctamin6; Anoctamin10; ANO1; ANO6; ANO10; Ca2+-activated Cl- channels; Retinal pigment epithelium; Primary cilium; Chloride secretion |
| Subjects: | 500 Science > 570 Life sciences |
| Divisions: | Biology, Preclinical Medicine > Institut für Physiologie Biology, Preclinical Medicine > Institut für Physiologie > Prof. Dr. Karl Kunzelmann |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 12 Apr 2019 12:27 |
| Last Modified: | 12 Apr 2019 12:27 |
| URI: | https://pred.uni-regensburg.de/id/eprint/4001 |
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