Stanewsky, Ralf and Lynch, Kathlea S. and Brandes, Christian and Hall, Jeffrey C. (2002) Mapping of elements involved in regulating normal temporal period and timeless RNA expression patterns in Drosophila melanogaster. JOURNAL OF BIOLOGICAL RHYTHMS, 17 (4). pp. 293-306. ISSN 0748-7304, 1552-4531
Full text not available from this repository. (Request a copy)Abstract
Although transcriptional regulation is a major force in generating circathan oscillations of clock molecules, posttranscriptional mechanisms also contribute to molecular rhythms. Applying novel transgenic period-luciferase constructs in transgenic Drosophila, the authors show that sequences within per's 5'-untranslated region mediate posttranscriptional regulation at the RNA level. Further mapping suggests that the relevant sequences for the correct phasing of period mRNA expression are located within the first intron. The results are consistent with a clock-regulated temporal stabilization of period mRNA during its daily upswing in the morning. This process is inferred to depend on a function of the PERIOD and TIMELESS proteins, and could further contribute to the observed delay between RNA and protein accumulation. Similarly, applying timeless-luciferase constructs led to the demonstration that regulatory elements for proper temporal timeless expression are present in a 4 kb promoter fragment and in sequences within the first intron. The results establish that, for normal rhythmicity, expression of clock genes requires regulation at the transcriptional, posttranscriptional, and posttranslational levels.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | CLOCK GENE; CIRCADIAN-RHYTHMS; POSTTRANSCRIPTIONAL REGULATION; TRANSCRIPTION; PROTEIN; PHOSPHORYLATION; CRYPTOCHROME; CONTRIBUTES; FEEDBACK; PROMOTER; Drosophila; circadian rhythms; period; timeless; luciferase; reporter transgenes; posttranscriptional regulation |
| Subjects: | 500 Science > 590 Zoological sciences |
| Divisions: | Biology, Preclinical Medicine > Institut für Zoologie |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 12 Oct 2021 06:23 |
| Last Modified: | 12 Oct 2021 06:23 |
| URI: | https://pred.uni-regensburg.de/id/eprint/40038 |
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