Gooch, K. J. and Blunk, T. and Courter, D. L. and Sieminski, A. L. and Vunjak-Novakovic, G. and Freed, L. E. (2002) Bone morphogenetic proteins-2, -12, and -13 modulate in vitro development of engineered cartilage. TISSUE ENGINEERING, 8 (4). pp. 591-601. ISSN 1076-3279
Full text not available from this repository. (Request a copy)Abstract
Bovine calf articular chondrocytes were seeded onto biodegradable polyglycolic acid (PGA) scaffolds and cultured in either control medium or medium supplemented with 1, 10, or 100 ng/mL of bone morphogenetic proteins (BMPs) BMP-2, BMP-12, or BMP-13. Under all conditions investigated, cell-polymer constructs cultivated for 4 weeks in vitro macroscopically and histologically resembled native cartilage. Addition of 100 ng/mL of BMP-2, BMP-12, or BMP-13 increased the total mass of the constructs relative to the controls by 121%, 80%, and 62%, respectively, which was accompanied by increases in the absolute amounts of collagen, glycosaminoglycans (GAG), and cells. The addition of 100 ng/mL of BMP-2, BMP-12, or BMP-13 increased the weight percentage of GAG in the constructs by 27%, 18%, and 15%, and decreased the weight percent of total collagen to 63%, 89%, and 83% of controls, respectively. BMP-2, but not BMP-12 or BMP-13 promoted chondrocyte hypertrophy. Taken together, these data suggest that BMP-2, BMP-12, and BMP-13 increase growth rate and modulate the composition of engineered cartilage and that 100 ng/mL of BMP-2 has the greatest effect. In addition, in vitro engineered cartilage provides a system for studying the effects of BMPs on chondrogenesis in a well-defined environment.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | INTERMITTENT PHYSIOLOGICAL PRESSURE; GROWTH-FACTOR; ARTICULAR CHONDROCYTES; REGENERATING CARTILAGE; MECHANICAL-PROPERTIES; ELASTIC CARTILAGE; POLYMER SCAFFOLDS; COLLAGEN GELS; IN-VITRO; TISSUE; |
| Subjects: | 600 Technology > 615 Pharmacy |
| Divisions: | Chemistry and Pharmacy > Institute of Pharmacy > Pharmaceutical Technology (Prof. Göpferich) |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 12 Oct 2021 08:56 |
| Last Modified: | 12 Oct 2021 08:56 |
| URI: | https://pred.uni-regensburg.de/id/eprint/40065 |
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