Weiss, Svenja and John, Gernot T. and Klimant, Ingo and Heinzle, Elmar (2002) Modeling of mixing in 96-well microplates observed with fluorescence indicators. BIOTECHNOLOGY PROGRESS, 18 (4): UNSP BP020. pp. 821-830. ISSN 8756-7938
Full text not available from this repository. (Request a copy)Abstract
Mixing in 96-well microplates was studied using soluble pH indicators and a fluorescence pH sensor. Small amounts of alkali were added with the aid of a multichannel pipet, a piston pump, and a piezoelectric actuator. Mixing patterns were observed visually using a video camera. Addition of drops each of about 1 nL with the piezoelectric actuator resulted in umbrella and double-disklike shapes. Convective mixing was mainly observed in the upper part of the well, whereas the lower part was only mixed quickly when using the multichannel pipet and the piston pump with an addition volume of 5 muL or larger. Estimated mixing times were between a few seconds and several minutes. Mixing by liquid dispensing was much more effective than by shaking. A mixing model consisting of 21 elements could describe mixing dynamics observed by the dissolved fluorescence dye and by the optical immobilized pH sensor. This model can be applied for designing pH control in microplates or for design of kinetic experiments with liquid addition.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | ROTARY SHAKING MACHINES; POWER-CONSUMPTION; UNBAFFLED FLASKS; LIQUID VISCOSITY; |
| Subjects: | 500 Science > 540 Chemistry & allied sciences |
| Divisions: | Chemistry and Pharmacy > Institut für Analytische Chemie, Chemo- und Biosensorik |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 18 Oct 2021 10:35 |
| Last Modified: | 18 Oct 2021 10:35 |
| URI: | https://pred.uni-regensburg.de/id/eprint/40098 |
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