Obermeier, Florian and Dunger, Nadja and Deml, Ludwig and Herfarth, Hans and Schoelmerich, Juergen and Falk, Werner (2002) CpG motifs of bacterial DNA exacerbate colitis of dextran sulfate sodium-treated mice. EUROPEAN JOURNAL OF IMMUNOLOGY, 32 (7). pp. 2084-2092. ISSN 0014-2980
Full text not available from this repository. (Request a copy)Abstract
Inflammatory bowel disease (IBD) is characterized by a dysregulated intestinal immune response with elevated levels of the. Th1 cytokines TNF, IL-12 and IFN-gamma. The luminal flora has been implicated as a major factor contributing to the initiation and perpetuation of chronic intestinal inflammation by as yet unknown mechanisms. Bacterial DNA contains unmethylated cytosine-guanosine dinucleotides (CpG) which strongly activate Th1-mediated immune responses. To test whether these CpG-motifs contribute to intestinal inflammation we treated mice with dextran-sulfate-sodium (DSS)-induced acute or chronic colitis for 5 days with CpG-containing oligodeoxynucleotides (CpG-ODN). Colonic inflammation was assessed by histological scoring. Colonic cytokine RNA was quantified by reverse transcription-PCR and cytokine secretion from mesenterial lymph node cells by ELISA. In chronic colitis, CpG-ODN treatment severely aggravated inflammation by 50%. Colonic expression of IFN-gamma and TNF was elevated (200- and 150-fold, respectively) and IFN-gamma and IL-12 secretion from lymph node cells was increased 5,000- and 8-fold, respectively, compared to GpG-ODN-treated controls. Similar effects were obtained in acute colitis. In conclusion, CpG-motifs; of bacterial DNA have proinflammatory activity by strengthening the Th1 arm of immunity in DSS-induced colitis, and might therefore play a significant role in the initiation and perpetuation of inflammation in IBD.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | RESIDENT INTESTINAL FLORA; TUMOR-NECROSIS-FACTOR; CROHNS-DISEASE; ENTERIC MICROFLORA; ULCERATIVE-COLITIS; MURINE COLITIS; T-CELLS; PERMEABILITY; INFLAMMATION; ACTIVATION; experimental colitis; dextran sulfate sodium; CpG motif; bacterial DNA |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Innere Medizin I Medicine > Lehrstuhl für Medizinische Mikrobiologie und Hygiene |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 18 Oct 2021 11:28 |
| Last Modified: | 18 Oct 2021 11:28 |
| URI: | https://pred.uni-regensburg.de/id/eprint/40109 |
Actions (login required)
 |
View Item |
