Schumacher, Karl and Strehl, Raimund and Minuth, Will W. (2002) Detection of glycosylated sites in embryonic rabbit kidney by lectin chemistry. HISTOCHEMISTRY AND CELL BIOLOGY, 118 (1). pp. 79-87. ISSN 0948-6143
Full text not available from this repository. (Request a copy)Abstract
The reciprocal cell biological interaction between mesenchymal and epithelial tissue plays a critical role during nophrogenesis. It is unknown to date whether the tissues interact during nephron induction by pure diffusion of substances or whether cellular contacts via gap junctions or focal adhesion molecules are involved. In neonatal rabbit kidney the interface between both tissues shows unique features. It consists of a distinct space, which is filled with specific extracellular matrix consisting of glycosylated proteins such as fibronectin, laminin, collagen, and proteoglycans. In the present experiments we tested by histochemistry whether it is possible to detect additional glycosylated proteins using Soybean agglutinin (SBA), Dolichos biflorus agglutinin (DBA), Ulex europaeus I agglutinin (UEA I), and Peanut agglutinin (PNA) as molecular markers. All tested lectins showed distinct labeling patterns in embryonic renal tissue. Within the collecting duct ampulla, DBA and UEA I revealed intensive cellular reaction. In contrast, PNA and SBA reacted at the basal aspect of the collecting duct ampulla tip in addition to a cellular reaction. To identify the individual molecules labeled by the lectins, embryonic tissue was fractionated and separated by electrophoretic methods. For the first time, we were able to show by two-dimensional electrophoresis and subsequent western blot experiments that lectins bind to a series of individual protein spots, which have not been identified to date.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | EPITHELIAL MESENCHYMAL INTERACTIONS; MOUSE KIDNEY; INTEGRIN ALPHA-8-BETA-1; NEPHROGENIC MESENCHYME; EXPRESSION; MORPHOGENESIS; DIFFERENTIATION; IDENTIFICATION; PROTEINS; CULTURE; kidney; development; lectins; histochemistry; two-dimensional electrophoresis |
| Subjects: | 500 Science > 570 Life sciences |
| Divisions: | Biology, Preclinical Medicine > Institut für Anatomie > Lehrstuhl für Molekulare und zelluläre Anatomie > Prof. Dr. Will Minuth |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 18 Oct 2021 11:37 |
| Last Modified: | 18 Oct 2021 11:37 |
| URI: | https://pred.uni-regensburg.de/id/eprint/40112 |
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