Bojak, A. and Wild, J. and Deml, L. and Wagner, Ralf (2002) Impact of codon usage modification on T cell immunogenicity and longevity of HIV-1 gag-specific DNA vaccines. INTERVIROLOGY, 45 (4-6). pp. 275-286. ISSN 0300-5526
Full text not available from this repository. (Request a copy)Abstract
In this study, we analyzed the in vitro expression, potency and longevity of immune responses induced in a Balb/ c mouse model by a synthetic HIV-1 gag gene exhibiting a codon usage that was adapted to that of highly expressed mammalian genes (syngag). In contrast to a vector containing the wild-type (wt) gag gene, the syngag construct enabled highly efficient Gag expression in both human and rodent cell lines in complete absence of Rev and Rev-responsive element. Immunization of Balb/c mice with the wt gag plasmid DNA induced only weak and inconsistent humoral immune responses. Mice vaccinated by syngag but not wt gag developed substantial and highly consistent Gag-specific antibody titers showing a clear T helper 1 polarization even with low doses of DNA. Moreover, vaccinated mice developed a strong Gag-specific cellular immune response, including cytotoxic T cells, which was not observed in wt gag-immunized animals. Both humoral and cellular immunity were efficient and lasted for more than 20 weeks. Furthermore, the induction of the humoral as well as the cellular immune response was independent of the immunization route (intramuscular or subcutaneous). These results clearly show the advantages of codon-optimized genes with respect to the expression and immunogenicity of plasmid DNA constructs, making them promising vaccine candidates for further studies. Copyright (C) 2003 S. Karger AG, Basel.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | IMMUNODEFICIENCY-VIRUS TYPE-1; LONG-TERM NONPROGRESSOR; LYMPHOCYTE ACTIVITY; IMMUNE-RESPONSES; PLASMID DNA; CTL EPITOPE; VIRAL-RNA; EXPRESSION; INFECTION; PROTEIN; HIV; synthetic genes; DNA vaccine; T cell immunogenicity |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Medizinische Mikrobiologie und Hygiene |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 18 Oct 2021 11:51 |
| Last Modified: | 18 Oct 2021 11:51 |
| URI: | https://pred.uni-regensburg.de/id/eprint/40116 |
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