Nagel, Georg and Ollig, Doris and Fuhrmann, Markus and Kateriya, Suneel and Mustl, Anna Maria and Bamberg, Ernst and Hegemann, Peter (2002) Channelrhodopsin-1: A light-gated proton channel in green algae. SCIENCE, 296 (5577). pp. 2395-2398. ISSN 0036-8075
Full text not available from this repository. (Request a copy)Abstract
Phototaxis and photophobic responses of green algae are mediated by rhodopsins with microbial-type chromophores. We report a complementary DNA sequence in the green alga Chlamydomonas reinhardtii that encodes a microbial opsin-related protein, which we term Channelopsin-1. The hydrophobic core region of the protein shows homology to the light-activated proton pump bacteriorhodopsin. Expression of Channelopsin-1, or only the hydrophobic core, in Xenopus laevis oocytes in the presence of all-trans retinal produces a light-gated conductance that shows characteristics of a channel selectively permeable for protons. We suggest that Channelrhodopsins are involved in phototaxis of green algae.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | VOLTAGE-DEPENDENCE; BACTERIORHODOPSIN; CHLAMYDOMONAS; RHODOPSIN; PHOTORECEPTOR; PHOTOTAXIS; RESOLUTION; PROTEIN; |
| Subjects: | 500 Science > 570 Life sciences |
| Divisions: | Biology, Preclinical Medicine > Institut für Biochemie, Genetik und Mikrobiologie |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 19 Oct 2021 06:15 |
| Last Modified: | 19 Oct 2021 06:15 |
| URI: | https://pred.uni-regensburg.de/id/eprint/40145 |
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