Grossmann, Johannes and Walther, Kathrin and Artinger, Monika and Ruemmele, Petra and Woenckhaus, Matthias and Schoelmerich, Juergen (2002) Induction of apoptosis before shedding of human intestinal epithelial cells. AMERICAN JOURNAL OF GASTROENTEROLOGY, 97 (6): PII S0002-. pp. 1421-1428. ISSN 0002-9270
Full text not available from this repository. (Request a copy)Abstract
OBJECTIVES: Human intestinal epithelial cells (IECs) derive from stem cells at the crypt base and migrate along the so-called crypt-villus axis toward the intestinal lumen. As they reach the luminal surface in the colon or the villus tip in the small intestine, IECs are shed and their life cycle is terminated. The role of apoptosis during IEC migration along the crypt-villus axis has been subject to studies with conflicting results. In this study we use a novel approach to identify the initiation of apoptosis within normal human IECs. METHODS: Normal mucosa from the large and small human intestine was analyzed employing a novel antibody directed against activated caspase-3-an early marker of apoptosis. RESULTS: IECs initiate the apoptotic cascade as they approach the area of shedding before displaying evident morphological features of apoptosis. IECs of the small bowel also show caspase-3 activation in the small intestinal crypt base, whereas IECs of the colononic crypt base rarely show evidence of ongoing apoptosis. CONCLUSIONS: These findings indicate that apoptosis is initiated in human IECs as they reach the luminal surface/villus tip and before shedding. Furthermore, they show that different sections of the intestinal tract vary significantly in the rate of IEC apoptosis, possibly reflecting their difference in susceptibility to epithelial cell neoplasia.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | IN-SITU DETECTION; DIFFERENTIAL EXPRESSION; GASTROINTESTINAL-TRACT; BCL-2 EXPRESSION; DEATH; IRRADIATION; CASPASE-3; PROTEASE; TENASCIN; TISSUES; |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Innere Medizin I Medicine > Lehrstuhl für Pathologie |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 19 Oct 2021 09:42 |
| Last Modified: | 19 Oct 2021 09:42 |
| URI: | https://pred.uni-regensburg.de/id/eprint/40167 |
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