Wolf, Michael and Lossdoerfer, Stefan and Marciniak, Jana and Roemer, Piero and Kirschneck, Christian and Craveiro, Rogerio and Deschner, James and Jaeger, Andreas (2016) CD8+T cells mediate the regenerative PTH effect in hPDL cells via Wnt10b signaling. INNATE IMMUNITY, 22 (8). pp. 674-681. ISSN 1753-4259, 1753-4267
Full text not available from this repository. (Request a copy)Abstract
It was the aim of the present investigation to examine whether the stimulating effect of parathyroid hormone (PTH) on human periodontal ligament (hPDL) cell proliferation and differentiation would be enhanced by hPDL/T-cell interaction involving Wnt10b signaling as a mediating pathway. hPDL cells were cultured from healthy premolar tissues of three adolescent orthodontic patients and exposed to PTH(1-34) in monocultures or co-cultures with CD8(+) T cells. At harvest, proliferation, alkaline phosphatase-specific activity (ALP), and osteocalcin production were determined by immunofluorescence cytochemistry, real-time PCR, biochemical assay, and ELISA. Wnt10b signaling was analyzed by the use of a specific WNT10b neutralizing antibody. PTH(1-34) stimulation of T cells significantly increased Wnt10b expression and production. Wnt10b exposure of hPDL cells enhanced proliferation and differentiation. PDL cells co-cultured with T cells showed a Wnt10b-dependent regulation of proliferation and differentiation parameters. The addition of a Wnt10b-neutralizing Ab to the co-culture medium resulted in a significant inhibition of the PTH(1-34) effect on proliferation, ALP-specific activity, and osteocalcin protein expression. Our findings provide novel insight into the mechanism of action of PTH on hPDL cells and establish the interplay of T cells and hPDL cells via the Wnt10b pathway as a modulating factor for the anabolic properties of the hormone in periodontal regeneration.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | PERIODONTAL-LIGAMENT CELLS; A-DEPENDENT PATHWAYS; PARATHYROID-HORMONE; IN-VITRO; OSTEOPROTEGERIN PRODUCTION; OVARIECTOMIZED RATS; BONE MASS; T-CELLS; DIFFERENTIATION; PTH(1-34); hPDL cells; immune cells; interaction; Wnt10b; proliferation; differentiation |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Zahnerhaltung und Parodontologie |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 12 Apr 2019 12:38 |
| Last Modified: | 12 Apr 2019 12:38 |
| URI: | https://pred.uni-regensburg.de/id/eprint/4019 |
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