Blindt, Ruediger and Bosserhoff, Anja-K. and Dahl, Jürgen vom and Hanrath, Peter and Schoer, Karsten and Hohlfeld, Thomas and Meyer-Kirchrath, Jutta (2002) Activation of IP and EP3 receptors alters cAMP-dependent cell migration. EUROPEAN JOURNAL OF PHARMACOLOGY, 444 (1-2): PII S0014-. pp. 31-37. ISSN 0014-2999
Full text not available from this repository. (Request a copy)Abstract
Migration of vascular smooth cells from the media to the intima essentially contributes to neointima formation after percutaneous transluminal angioplasty and stent implantation. The stable prostacyclin mimetic iloprost has been shown to inhibit neointima formation in experimental restenosis, but it is currently unknown whether this may be caused by an antimigratory effect. Hence, the present study analyses (i) the influence of G(s)-coupled prostacyclin (IP) receptors on cell migration and (ii) verifies whether EP3 receptors with opposite (i.e., G(i)) coupling may conversely stimulate cell migration. In a modified Boyden chamber model, it was shown that iloprost dose-dependently inhibits the migration of primary human arterial smooth muscle cells, which constitutively express the IP receptor. On the other hand, human arterial smooth muscle cell migration was stimulated by the EP3 receptor agonist M&B 28.767. To independently study the effects of these receptors, IP or EP3 receptors were stably overexpressed in chinese hamster ovary cells (CHO-IP and CHO-EP3). Chemotaxis of CHO cells transfected with G(s)-coupled IP receptors was concentration-dependently inhibited by iloprost (2-100 nM), while there was no effect of iloprost on mock-transfected CHO, By contrast, CHO-cells that overexpressed EP3 receptors showed a significant, concentration dependent (1-100 nM) increase of cell migration in presence of the selective ER, agonist M&B 28.767. It is concluded that the prostacyclin mimetic iloprost inhibits vascular cell migration, which probably depends on a G(s)-mediated increase of intracellular cAMP. EP3 receptors conversely stimulate CHO migration. (C) 2002 Elsevier Science B.V. All rights reserved.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | HAMSTER OVARY CELLS; CORONARY ANGIOPLASTY; NEOINTIMA FORMATION; BALLOON INJURY; GENE-TRANSFER; PROSTACYCLIN; EXPRESSION; DESENSITIZATION; INHIBITION; RESTENOSIS; IP receptor; EP3 receptor; cAMP; cell migration |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Pathologie |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 26 Oct 2021 07:05 |
| Last Modified: | 26 Oct 2021 07:05 |
| URI: | https://pred.uni-regensburg.de/id/eprint/40243 |
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