Buechler, Christa and Boettcher, Alfred and Bared, Salim Maa and Probst, Mario C. O. and Schmitz, Gerd (2002) The carboxyterminus of the ATP-binding cassette transporter A1 interacts with a beta 2-syntrophin/utrophin complex. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 293 (2): PII S0006-. pp. 759-765. ISSN 0006-291X
Full text not available from this repository. (Request a copy)Abstract
Recent work identified ABCA1 as the major regulator of plasma HDL-cholesterol responsible for the removal of excess cholinephospholipids and cholesterol from peripheral cells and tissues. ABCA1 function may depend on the association with heteromeric proteins and to identify these candidates a human liver yeast two-hybrid library was screened with the carboxyterminal 144 amino acids of ABCA1, beta2-Syntrophin was found to interact with ABCA1 and the C-terminal five amino acids of ABCA1 proned to represent a perfect tail for binding to syntrophin PDZ domains. Immunoprecipitation further confirmed the association of ABCA1 and beta2-syntrophin and in addition utrophin, known to couple beta2-syntrophin and its PDZ ligands to the F-actin cytoskeleton, was identified as a constituent of this complex. ABCA1 in the plasmamembrane of human macrophages was found to be partially associated with Lubrol rafts and effluxed choline-phospholipids involve these microdomains. beta2-Syntrophin does not colocalize in these rafts indicating that beta2-syntrophin may participate in the retaining of ABCA1 in cytoplasmic vesicles and for the targeting of ABCA1 to plasmamembrane microdomains when ABCA1 is released from beta2-syntrophin. (C) 2002 Elsevier Science (USA). All rights reserved.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | TANGIER-DISEASE; PDZ DOMAINS; CHOLESTEROL EFFLUX; STARGARDT-DISEASE; HUMAN ABCA1; GENE; PROTEIN; MUTATIONS; DYSTROPHIN; MECHANISM; ABCA1; beta 2-syntrophin; utrophin; actin; PDZ-domain |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Klinische Chemie und Laboratoriumsmedizin |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 26 Oct 2021 09:57 |
| Last Modified: | 26 Oct 2021 09:57 |
| URI: | https://pred.uni-regensburg.de/id/eprint/40270 |
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