Kuerner, Jens M. and Wolfbeis, Otto S. and Klimant, Ingo (2002) Homogeneous luminescence decay time-based assay using energy transfer from nanospheres. ANALYTICAL CHEMISTRY, 74 (9). pp. 2151-2156. ISSN 0003-2700, 1520-6882
Full text not available from this repository. (Request a copy)Abstract
Following a study on the feasibility of resonance energy transfer (RET) from carboxylated nanospheres with an incorporated phosphorescent donor to a cationic polyelectrolyte/acceptor aggregate on their surface, a novel scheme for homogeneous assays is presented that is based on RET from phosphorescent biotinylated nanospheres to fluorescently labeled streptavidin (SA). The phosphorescent nanospheres, with a diameter of well below 50 nm, are made from carboxylated polyacrylonitrile and dyed with ruthenium(II)-tris-4,7-diphenyl-1,10-phenanthroline dichloride (Ru(dpp)). Due to the small size of the nanospheres and the complete extraction of the ruthenium dye into the nanospheres during the precipitation process, RET occurs from Ru(dpp) to the label if labeled SA binds to the surface of the nanospheres. Luminescence quenching by oxygen or other species present in the sample can be neglected due to the shielding effect of the polymer matrix. Based on this finding, a competitive binding assay was established, where avidin and labeled SA compete for the biotin binding sites on the nanosphere. The process of binding to the surface can be detected by measurement of the luminescence intensity or the apparent decay time which is in the order of 2.5-4.5 mus.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | PHOSPHORESCENT NANOSPHERES; COMPLEXES; |
| Subjects: | 500 Science > 540 Chemistry & allied sciences |
| Divisions: | Chemistry and Pharmacy > Institut für Analytische Chemie, Chemo- und Biosensorik > Chemo- und Biosensorik (Prof. Antje J. Bäumner, formerly Prof. Wolfbeis) |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 26 Oct 2021 10:09 |
| Last Modified: | 26 Oct 2021 10:09 |
| URI: | https://pred.uni-regensburg.de/id/eprint/40276 |
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