Hartwich, Jadwiga and Dembinska-Kiec, Aldona and Gruca, Anna and Motyka, Marcin and Partyka, Lukasz and Skrzeczynska, Joanna and Bzowska, Malgorzata and Pryjma, Juliusz and Huber, Joakim and Leitinger, Norbert and Schmitz, Gerd (2002) Regulation of platelet adhesion by oxidized lipoproteins and oxidized phospholipids. PLATELETS, 13 (3). pp. 141-151. ISSN 0953-7104
Full text not available from this repository. (Request a copy)Abstract
Activated platelets adhere to the endothelium and release vasoactive mediators which induce vasoconstriction and remodeling of the vessel wall. The influence of native and ex vivo oxidized lipoproteins enriched with oxidized 1-palmitoyl-2-arachidonoyl-sn-glycero-3-phosphorylcholine (ox-PAPC), the major lipid responsible for the biological activity of minimally oxidized LDL (mm-LDL), on platelet adhesion, membrane receptor expression and aggregation was studied. Influence of native and oxidized lipoproteins (5-100 mug protein/ml); ox-PAPC (0.5-50 mug/ml); ADP (1-10 muM) as well as the specific phosphatase 1 and 2A inhibitor okadaic acid (3-10 muM) on platelet adhesion, receptor expression and aggregation was measured. Platelets adhered to all the classes of lipoproteins immobilized in plastic microtiter wells (native lipoproteins: HDL< LDL< VLDL< oxidized lipoproteins<ox-PAPC-enriched lipoproteins). Flow cytometry revealed that lipoproteins increased CD41 expression. Preincubation of platelets with ox-PAPC alone, significantly up-regulated CD62p and CD41 receptors (higher dose) but potently inhibited anti-CD36 MoAb binding. Okadaic acid increased anti-CD41 and decreased anti-CD36 and anti-CD42b MoAbs binding. Neither ox-PAPC nor okadaic acid induced platelet aggregation. CD36 seems to be the main receptor responsible for binding of oxidized lipoproteins, particularly its ox-PAPC epitope. The effect of okadaic acid on CD36 and CD41 argue for the participation of phosphorylation-dependent reorganization of cellular trafficking and microtubule organization by ox-PAPC.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | LOW-DENSITY-LIPOPROTEIN; AORTIC ENDOTHELIAL-CELLS; OKADAIC ACID; ATHEROSCLEROSIS; AGGREGATION; BINDING; CD36; PHOSPHATASES; PARAOXONASE; ACTIVATION; |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Klinische Chemie und Laboratoriumsmedizin |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 27 Oct 2021 15:24 |
| Last Modified: | 27 Oct 2021 15:24 |
| URI: | https://pred.uni-regensburg.de/id/eprint/40364 |
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