Heinz, Sven and Krause, Stefan W. and Gabrielli, Franco and Wagner, Harald M. and Andreesen, Reinhard and Rehli, Michael (2002) Genomic organization of the human gene HEP27: Alternative promoter usage in HepG2 cells and monocyte-derived dendritic cells. GENOMICS, 79 (4). pp. 608-615. ISSN 0888-7543, 1089-8646
Full text not available from this repository. (Request a copy)Abstract
We used representational difference analysis to discover new genes with specific expression in dendritic cells. Among other genes, we identified HEP27, encoding a member of the short chain alcohol dehydrogenase/reductase family to be upregulated during monocyte to dendritic cell differentiation. Originally cloned from hepatocellular carcinoma cells (HepG2), HEP27 was exclusively expressed in monocyte-derived dendritic cells within the hematopoietic system. The presence of different transcripts in monocyte-derived dendritic cells, HepG2 cells, and various tissues could be traced back to alternative splicing and alternative promoter usage. We describe here the complete genomic organization of HEP27, including two alternative promoter regions: a hepatocyte-specific promoter which was induced by the histone deacetylase inhibitor sodium butyrate in several other cell types, and a second upstream promoter which was specifically active in monocyte-derived dendritic cells. Its exclusive usage in monocyte-derived dendritic cells makes the alternative HEP27 promoter an interesting target to study dendritic-cell-specific gene regulation.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | NUCLEAR-PROTEIN; BLOOD MONOCYTES; EXPRESSION; IDENTIFICATION; DIFFERENTIATION; TRANSCRIPTION; METHYLATION; ACTIVATION; PHENOTYPE; REGION; alcohol dehydrogenase; dendritic cells; carcinoma; hepatocellular; promoter regions (genetics); exons; RNA splicing; endogenous retroviruses; butyric acid; histone deacetylase |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Innere Medizin III (Hämatologie und Internistische Onkologie) |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 02 Nov 2021 13:35 |
| Last Modified: | 02 Nov 2021 13:35 |
| URI: | https://pred.uni-regensburg.de/id/eprint/40415 |
Actions (login required)
 |
View Item |
