Straub, Rainer H. and Pongratz, Georg and Guenzler, Christian and Michna, Andreas and Baier, Simone and Kees, Frieder and Falk, Werner and Schoelmerich, Juergen (2002) Immunoregulation of IL-6 secretion by endogenous and exogenous adenosine and by exogenous purinergic agonists in splenic tissue slices. JOURNAL OF NEUROIMMUNOLOGY, 125 (1-2): PII S0165-. pp. 73-81. ISSN 0165-5728, 1872-8421
Full text not available from this repository. (Request a copy)Abstract
In recent years, the role of norepinephrine, opioids, and neuropeptide Y for sympathetic regulation of murine spleen cells has been characterised. In this study, we describe the role of exogenous and endogenous adenosine and exogenous P2X(1) and P2Y(1) agonists for spontaneous splenic IL-6 secretion from spleen slices. The P2X(1) agonist beta,gamma-methylene ATP inhibited IL-6 secretion at 10(-5) M, whereas the P2Y(1) agonist 2-methylthio ATP increased IL-6 secretion at 10(-6) to 10(-8) M. Furthermore, adenosine (at 5 x 10(-8), 10(-7), 5 x 10(-7) M) inhibited IL-6 secretion via Al adenosine receptors, whereas an A2(A) adenosine receptor agonist increased IL-6 secretion in the presence of 10(-7) M cortisol. To determine the effects of endogenous adenosine, electrical field stimulation was applied in order to release endogenous ATP, which yields adenosine after conversion from ATP. Electrical field stimulation markedly reduced IL-6 secretion, which was attenuated by the Al antagonist DPCPX but not by the A2 antagonist 8-(3-Chlorostyryl)caffeine. Thus, via Al adenosine receptors, adenosine was found to be a strong inhibitor of splenic IL-6 secretion. This study further expands our earlier description of the complexity of the local dialogue of sympathetic nerves and macrophages in lymphoid organs. (C) 2002 Elsevier Science B.V. All rights reserved.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | GLUCOCORTICOID-RECEPTOR; INTERLEUKIN-6 SECRETION; DEAMINASE DEFICIENCY; MESSENGER-RNA; IMMUNE-SYSTEM; MURINE SPLEEN; COMBINED IMMUNODEFICIENCY; DIFFERENTIAL REGULATION; SYMPATHETIC-NERVES; PROTEIN-SYNTHESIS; ATP; adenosine; sympathetic nerve; splenic macrophage; IL-6 |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Innere Medizin I |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 03 Nov 2021 07:40 |
| Last Modified: | 03 Nov 2021 07:40 |
| URI: | https://pred.uni-regensburg.de/id/eprint/40436 |
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