Schultz, Michael and Veltkamp, Claudia and Dieleman, Levinus A. and Grenther, Wetonia B. and Wyrick, Pricilla B. and Tonkonogy, Susan L. and Sartor, R. Balfour (2002) Lactobacillus plantarum 299V in the treatment and prevention of spontaneous colitis in interleukin-10-deficient mice. INFLAMMATORY BOWEL DISEASES, 8 (2). pp. 71-80. ISSN 1078-0998, 1536-4844
Full text not available from this repository. (Request a copy)Abstract
lnterleukin (IL)-10-deficient (IL-10(-/-)) mice develop colitis under specific pathogen-free (SPF) conditions and remain disease free if kept sterile (germ free [GF]). We used four different protocols that varied the time-points of oral administration of Lactobacillus plantarian 299v (L. plantarian) relative to colonization with SPF bacteria to determine whether L. plantarum could prevent and treat colitis induced by SPF bacteria in IL-10(-/-) mice and evaluated the effect of this probiotic organism on mucosal immune activation. Assessment of colitis included blinded histologic scores, measurements of secreted colonic immunoglobulin isotypes, IL-12 (p40 subunit), and interferon (IFN)-gamma production by anti-CD3-stimulated mesenteric lymph node cells. Treating SPF IL-10(-/-) mice with L. plantarum attenuated previously established colonic inflammation as manifested by decreased mucosal IL-12, IFTN-gamma, and immunoglobulin 02, levels. Colonizing GF animals with L. plantarum and SPF flora simultaneously had no protective effects. Gnotobiotic IL-10(-/-) mice monoassociated with L. plantarian exhibited mild immune system activation but no colitis. Pretreatment of GF mice by colonization with L. plantarum, then exposure to SPF flora and continued probiotic therapy significantly decreased histologic colitis scores. These results demonstrate that L. plantarum can attenuate immune-mediated colitis and suggest a potential therapeutic role for this agent in clinical inflammatory bowel diseases.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | INFLAMMATORY-BOWEL-DISEASE; IMMUNE-SYSTEM ACTIVATION; PLACEBO-CONTROLLED TRIAL; NECROSIS-FACTOR-ALPHA; GENE-DEFICIENT MICE; ULCERATIVE-COLITIS; ESCHERICHIA-COLI; DOUBLE-BLIND; MAINTENANCE TREATMENT; DEVELOP COLITIS; probiotics; experimental colitis; interleukin-10 knockout mice |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Innere Medizin I |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 08 Nov 2021 15:46 |
| Last Modified: | 08 Nov 2021 15:46 |
| URI: | https://pred.uni-regensburg.de/id/eprint/40512 |
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