Boehmer, Martin and Wahl, Michael and Rahn, Hans-Jürgen and Erdmann, Rainer and Enderlein, Joerg (2002) Time-resolved fluorescence correlation spectroscopy. CHEMICAL PHYSICS LETTERS, 353 (5-6): PII S0009-. pp. 439-445. ISSN 0009-2614, 1873-4448
Full text not available from this repository. (Request a copy)Abstract
A new method of performing fluorescence correlation spectroscopy (FCS) measurements for mixtures of several fluorescent molecular species is introduced. It uses time-resolved fluorescence detection for separating the different FCS-contributions from the different species. This allows simultaneous and independent monitoring of the diffusion of several molecular species in one sample, or performing multi-label cross-correlation measurements. In this way, the proposed method is equivalent to dual- or multi-color FCS. However, it is simpler to implement experimentally, because it requires only single wavelength excitation and detection. This Letter outlines the theoretical basis and presents experimental results of the method. (C) 2002 Elsevier Science B.V. All rights reserved.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | CROSS-CORRELATION SPECTROSCOPY; SINGLE-MOLECULE DETECTION; BIOTECHNOLOGY; DIAGNOSTICS; |
| Subjects: | 500 Science > 540 Chemistry & allied sciences |
| Divisions: | Chemistry and Pharmacy > Institut für Analytische Chemie, Chemo- und Biosensorik > Chemo- und Biosensorik (Prof. Antje J. Bäumner, formerly Prof. Wolfbeis) |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 09 Nov 2021 07:37 |
| Last Modified: | 09 Nov 2021 07:37 |
| URI: | https://pred.uni-regensburg.de/id/eprint/40577 |
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