Tang, Thean Hock and Rozhdestvensky, Timofey S. and d'Orval, Beatrice Clouet and Bortolin, Marie-Line and Huber, Harald and Charpentier, Bruno and Branlant, Christiane and Bachellerie, Jean-Pierre and Brosius, Juergen and Huttenhofer, Alexander (2002) RNomics in Archaea reveals a further link between splicing of archaeal introns and rRNA processing. NUCLEIC ACIDS RESEARCH, 30 (4). pp. 921-930. ISSN 0305-1048, 1362-4962
Full text not available from this repository. (Request a copy)Abstract
The bulge-helix-bulge (BHB) motif recognised by the archaeal splicing endonuclease is also found in the long processing stems of archaeal rRNA precursors in which it is cleaved to generate pre-16S and pre-23S rRNAs. We show that in two species, Archaeoglobus fulgidus and Sulfolobus solfataricus, representatives from the two major archaeal kingdoms Euryarchaeota and Crenarchaeota, respectively, the pre-rRNA spacers cleaved at the BHB motifs surrounding pre-16S and pre-23S rRNAs subsequently become ligated. In addition, we present evidence that this is accompanied by circularisation of ribosomal pre-16S and pre-23S rRNAs in both species. These data reveal a further link between intron splicing and pre-rRNA processing in Archaea, which might reflect a common evolutionary origin of the two processes. One spliced RNA species designated 16S-D RNA, resulting from religation at the BHB motif of 16S pre-rRNA, is a highly abundant and stable RNA which folds into a three-stem structure interrupted by two single-stranded regions as assessed by chemical probing. It spans a region of the pre-rRNA 5' external transcribed spacer exhibiting a highly conserved folding pattern in Archaea. Surprisingly, 16S-D RNA contains structural motifs found in archaeal C/D box small RNAs and binds to the L7Ae protein, a core component of archaeal C/D box RNPs. This supports the notion that it might have an important but still unknown role in pre-rRNA biogenesis or might even target RNA molecules other than rRNA.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | SMALL NUCLEOLAR RNAS; U6 SPLICEOSOMAL RNA; RIBOSOMAL-RNA; SULFOLOBUS-ACIDOCALDARIUS; 2'-O-RIBOSE METHYLATION; HALOBACTERIUM-VOLCANII; ENDONUCLEASE; SEQUENCES; SUBSTRATE; ARCHAEBACTERIA; |
| Subjects: | 500 Science > 570 Life sciences |
| Divisions: | Biology, Preclinical Medicine > Institut für Biochemie, Genetik und Mikrobiologie > Lehrstuhl für Mikrobiologie (Archaeenzentrum) |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 09 Nov 2021 09:53 |
| Last Modified: | 09 Nov 2021 09:53 |
| URI: | https://pred.uni-regensburg.de/id/eprint/40592 |
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