Scheide, Dierk and Huber, Robert and Friedrich, Thorsten (2002) The proton-pumping NADH : ubiquinone oxidoreductase (complex 1) of Aquifex aeolicus. FEBS LETTERS, 512 (1-3): PII S0014-. pp. 80-84. ISSN 0014-5793
Full text not available from this repository. (Request a copy)Abstract
The proton-pumping NADH:ubiquinone oxidoreductase, also called complex 1, is the first energy-transducing complex of many respiratory chains. Homologues of complex I are present in the three domains of life. Here. we report the properties of complex I in membranes of the hyperthermophilic bacterium Aquifex aeolicus. The complex reacted with NADH but not with NADPH and F420H2 as electron donors. Short-chain analogues of ubiquinone like decyl-ubiquinone and ubiquinone-2 were suitable electron acceptors. The affinities towards NADH and ubiquinone-2 were comparable to the ones obtained with the Escherichia coli complex I. The reaction was inhibited by piericidin A at the same concentration as in E. coli. The complex showed an unusual pH optimum at pH 9 and a maximal rate at 80degreesC. We found no evidence for the presence of an alternative. single subunit NADH dehydrogenase in A. aeolicus membranes. The NADH:ferricyanide reductase activity of detergent extracts of A. aeolicus membranes sedimented as a protein with a molecular mass of approximately 550 kDa. From the data Ne concluded that A. acolicus contains a NADH: ubiquinone oxidoreductase resembling complex I of mesophilic bacteria. (C) 2002 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | RESPIRATORY-CHAIN NADH; ESCHERICHIA-COLI; 3-DIMENSIONAL STRUCTURE; QUINONE OXIDOREDUCTASE; DEHYDROGENASE; BACTERIAL; MITOCHONDRIA; EVOLUTION; SEQUENCE; GENOME; complex I; NADH : ubiquinone oxidoreductase; NADH dehydrogenase; extremophile; hyperthermophile; Aquifex aeolicus |
| Subjects: | 500 Science > 570 Life sciences |
| Divisions: | Biology, Preclinical Medicine > Institut für Biochemie, Genetik und Mikrobiologie > Lehrstuhl für Mikrobiologie (Archaeenzentrum) |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 09 Nov 2021 10:03 |
| Last Modified: | 09 Nov 2021 10:03 |
| URI: | https://pred.uni-regensburg.de/id/eprint/40595 |
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