Grimm, Daniela and Bauer, Johann and Kossmehl, Peter and Shakibaei, Mehdi and Schoenberger, Johann and Pickenhahn, Holger and Schulze-Tanzil, Gundula and Vetter, Roland and Eilles, Christoph and Paul, Martin and Cogoli, Augusto (2002) Simulated microgravity alters differentiation and increases apoptosis in human follicular thyroid carcinoma cells. FASEB JOURNAL, 16 (2). 604-+. ISSN 0892-6638
Full text not available from this repository. (Request a copy)Abstract
This study focuses on the effects of simulated microgravity (0g) on the human follicular thyroid carcinoma cell line ML-1. Cultured on a three-dimensional clinostat, ML-1 cells formed three-dimensional MCTSs (MCTS diameter: 0.3+/-0.01 mm). After 24 and 48 h of clinorotation, the cells significantly decreased fT3 and fT4 secretion but up-regulated the thyroid-stimulating hormone-receptor expression as well as the production of vimentin, vinculin, and extracellular matrix proteins (collagen I and III, laminin, fibronectin, chondroitin sulfate) compared with controls. Furthermore, ML-1 cells grown on the clinostat showed elevated amounts of the apoptosis-associated Fas protein, of p53, and of bax but showed reduced quantities of bcl-2. In addition, signs of apoptosis became detectable, as assessed by terminal deoxynucleotidyl transferase-mediated dUTP digoxigenin nick end labeling, 4', 6-diamidino-2-phenylindole staining, DNA laddering, and 85-kDa apoptosis-related cleavage fragments. These fragments resulted from enhanced 116-kDa poly(ADP-ribose)polymerase (PARP) activity and apoptosis. These observations suggest that clinorotation elevates intermediate filaments, cell adhesion molecules, and extracellular matrix proteins and simultaneously induces apoptosis in follicular thyroid cancer cells. In conclusion, our experiments could provide a regulatory basis for the finding that astronauts show low thyroid hormone levels after space flight, which may be explained by the increase of apoptosis in thyrocytes as a result of simulated 0g.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | EXTRACELLULAR-MATRIX PROTEINS; MULTICELLULAR TUMOR SPHEROIDS; POLY(ADP-RIBOSE) POLYMERASE; MONOLAYER-CULTURES; PRESSURE-OVERLOAD; GENE-EXPRESSION; GROWTH; SPACE; SPACEFLIGHT; DEATH; extracellular matrix proteins; hormones; clinorotation; thyroid tumor; programmed cell death |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Abteilung für Nuklearmedizin |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 09 Nov 2021 13:10 |
| Last Modified: | 09 Nov 2021 13:10 |
| URI: | https://pred.uni-regensburg.de/id/eprint/40617 |
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