Mansky, Kim C. and Sulzbacher, Sabine and Purdom, Georgia and Nelsen, Lori and Hume, David A. and Rehli, Michael and Ostrowski, Michael C. (2002) The microphthalmia transcription factor and the related helix-loop-helix zipper factors TFE-3 and TFE-C collaborate to activate the tartrate-resistant acid phosphatase promoter. JOURNAL OF LEUKOCYTE BIOLOGY, 71 (2). pp. 304-310. ISSN 0741-5400
Full text not available from this repository.Abstract
The microphthalmia transcription factor (MITF) regulates different target genes in several distinct cell types, including osteoclasts. The role of the closely related factors TFE3 and TFEC in MITF action was studied. The TFE3 and TFEC proteins were expressed in osteoclast-like cells, and both could be immunoprecipitated in a complex with MITF. In transient transfection assays, TFE3 and TFEC could collaborate with MITF to superactivate the tartrate resistant acid phosphatase (TRAP) promoter, a target for MITF in osteoclasts. Although TFEC had been thought to act as a repressor, we could demonstrate that TFEC acted as a transactivator when fused to the gal4 DNA-binding domain in a yeast one-hybrid-type assay. Additionally, two mRNA isoforms of MITF, MITF-M and MITF-A, were detected in primary osteoclast-like cells by RT-PCR. In transient transfection assays, the MITF-A and MITF-M isoforms activated the promoter of the TRAP gene to the same extent, and both forms could collaborate equally well with TFE3 to activate the TRAP promoter. These results indicate that although different isoforms of MITF appear to he functionally similar, the TFE3 and TFEC proteins may collaborate with MITF to efficiently regulate expression of target genes in osteoclasts.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | OSTEOCLAST DIFFERENTIATION; MITF GENE; MOUSE; MUTATIONS; PROTEIN; LOCUS; PHOSPHORYLATION; SUBFAMILY; myeloid differentiation; protein dimerization |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Innere Medizin III (Hämatologie und Internistische Onkologie) |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 10 Nov 2021 14:42 |
| Last Modified: | 10 Nov 2021 14:42 |
| URI: | https://pred.uni-regensburg.de/id/eprint/40633 |
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