Blunk, Torsten and Sieminski, Alisha L. and Gooch, Keith J. and Courter, Donald L. and Hollander, Anthony P. and Nahir, A. Menahem and Langer, Robert and Vunjak-Novakovic, Gordana and Freed, Lisa E. (2002) Differential effects of growth factors on tissue-engineered cartilage. TISSUE ENGINEERING, 8 (1). pp. 73-84. ISSN 1076-3279
Full text not available from this repository.Abstract
The effects of four regulatory factors on tissue-engineered cartilage were examined with specific focus on the ability to increase construct growth rate and concentrations of glycosaminoglycans (GAG) and collagen, the major extracellular matrix (ECM) components. Bovine calf articular chondrocytes were seeded onto biodegradable polyglycolic acid (PGA) scaffolds and cultured in medium with or without supplemental insulin-like growth factor (IGF-I), interleukin-4 (IL-4), transforming growth factor-beta1 (TGF-beta1) or platelet-derived growth factor (PDGF). IGF-I, IL-4, and TGF-beta1 increased construct wet weights by 1.5-2.9-fold over 4 weeks of culture and increased amounts of cartilaginous ECM components. IGF-I (10-300 ng/mL) maintained wet weight fractions of GAG in constructs seeded at high cell density and increased by up to fivefold GAG fractions in constructs seeded at lower cell density. TGF-beta1 (30 ng/mL) increased wet weight fractions of total collagen by up to 1.4-fold while maintaining a high fraction of type II collagen (79 +/- 11% of the total collagen). IL-4 (1-100 ng/mL) minimized the thickness of the GAG-depleted region at the construct surfaces. PDGF (1-100 ng/mL) decreased construct growth rate and ECM fractions. Different regulatory factors thus elicit significantly different chondrogenic responses and can be used to selectively control the growth rate and improve the composition of engineered cartilage.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | ARTICULAR-CARTILAGE; II COLLAGEN; PROTEOGLYCAN METABOLISM; POLYMER SCAFFOLDS; MATRIX SYNTHESIS; CHONDROCYTES; CULTURES; EXPLANTS; INTERLEUKIN-4; TGF-BETA-1; |
| Subjects: | 600 Technology > 615 Pharmacy |
| Divisions: | Chemistry and Pharmacy > Institute of Pharmacy > Pharmaceutical Technology (Prof. Göpferich) |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 15 Nov 2021 08:12 |
| Last Modified: | 15 Nov 2021 08:12 |
| URI: | https://pred.uni-regensburg.de/id/eprint/40661 |
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