Harangi, Mariann and Aslanidis, Charalampos and Paragh, Gyoergy and Schmitz, Gerd (2002) High-speed detection of the two common paraoxonase polymorphisms Leu(55)-> Met and Gln(192)-> Arg by real-time fluorescence PCR and melting curves. CLINICAL CHEMISTRY AND LABORATORY MEDICINE, 40 (4). pp. 337-340. ISSN 1434-6621
Full text not available from this repository. (Request a copy)Abstract
Human paraoxonase (PON1) is a calcium-dependent esterase exclusively bound to apolipoprotein A-I and clusterin, containing high-density lipoprotein (HDL) particles that hydrolyzes organophosphates and aryl esters. Several studies have indicated that PON1 can prevent low-density lipoprotein (LDL) oxidation by hydrolyzing lipid peroxides in the lipoprotein, which is the crucial first step for atherogenesis. Therefore it may protect against the development of atherosclerosis. Serum PON1 activity has been shown to be decreased in familiar hypercholesterolemia and in diseases that are associated with accelerated atherogenesis. The PON1 gene has two common coding region polymorphisms, Leu(55)-->Met and Gln(192) -->Arg. Both polymorphisms have been identified as independent risk factors for cardiovascular disease in diabetic and non-diabetic patients. We have established high-speed and easy-to-perform genotyping for the two most significant PON1 gene polymorphisms, employing the LightCycler technology and melting curves. This technique eliminates PCR contamination related to sample handling and does not require digestion of PCR products with restriction enzymes and/or fragment separation on gels.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | DENSITY-LIPOPROTEIN; SERUM; DISEASE; paraoxonase; real-time fluorescence genotyping; single nucleotide polymorphisms |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Klinische Chemie und Laboratoriumsmedizin |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 15 Nov 2021 14:53 |
| Last Modified: | 15 Nov 2021 14:53 |
| URI: | https://pred.uni-regensburg.de/id/eprint/40718 |
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