del Barrio, Melisa and Cases, Rafael and Cebolla, Vicente and Hirsch, Thomas and de Marcos, Susana and Wilhelm, Stefan and Galban, Javier (2016) A reagentless enzymatic fluorescent biosensor for glucose based on upconverting glasses, as excitation source, and chemically modified glucose oxidase. TALANTA, 160. pp. 586-591. ISSN 0039-9140, 1873-3573
Full text not available from this repository. (Request a copy)Abstract
Upon near-infrared excitation Tm3++Yb3+ doped fluorohafnate glasses present upconversion properties and emit visible light. This property permits to use these glasses (UCG) as excitation sources for fluorescent optical biosensors. Taking this into account, in this work a fluorescent biosensor for glucose determination is designed and evaluated. The biosensor combines the UCG and the fluorescence of the enzyme glucose oxidase chemically modified with a fluorescein derivative (GOx-FS), whose intensity is modified during the enzymatic reaction with glucose. Optical parameters have been optimized and a mathematical model describing the behavior of the analytical signal is suggested. Working in FIA mode, the biosensor responds to glucose concentrations up to, at least, 15 mM with a limit of detection of 1.9 mM. The biosensor has a minimum lifetime of 9 days and has been applied to glucose determination in drinks. The applicability of the sensor was tested by glucose determination in two fruit juices. (C) 2016 Elsevier B.V. All rights reserved.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | NANOPARTICLES; PROTEINS; Upconverting glasses; Glucose Oxidase; Glucose Determination; Inner Filter Effect |
| Subjects: | 500 Science > 540 Chemistry & allied sciences |
| Divisions: | Chemistry and Pharmacy > Institut für Analytische Chemie, Chemo- und Biosensorik |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 24 Apr 2019 09:15 |
| Last Modified: | 24 Apr 2019 09:15 |
| URI: | https://pred.uni-regensburg.de/id/eprint/4074 |
Actions (login required)
 |
View Item |
