Sizova, I. A. and Hegemann, Peter and Furmann, M. and Danilenko, V. N. (2002) Streptomyces rimosus aminoglycoside 3 '-phosphotransferase VIII: Comparisons with aminoglycoside 3 '-phosphotransferases of aminoglycoside-producing strains and with eukaryotic protein kinases. MOLECULAR BIOLOGY, 36 (1). pp. 18-25. ISSN 0026-8933, 1608-3245
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The nucleotide sequence was established for the aphVIII aminoglycoside phosphotransferase gene of an oxytetracycline-producing Streptomyces rimosus strain. The gene is 804 bp in size and possibly codes for APHVIII of 267 residues. Heterologous expression of aphVIII was studied in Escherichia coli and Chlamy-domonas reinhardtii. The deduced APHVIII sequence was compared with known sequences of aminoglycoside phosphotransferases of aminoglycoside-producing actinomycete strains and of eukaryotic protein kinases. A local homology of 38 residues was found between APHVIII and actinomycete serine-threonine protein kinases in the conserved region possibly involved in ATP binding. APHVIII differed from aminoglycoside 3'-phosphotransferases of aminoglycoside-producing actinomycete strains and of clinical isolates, and can be classed to a separate group.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | SERINE THREONINE KINASE; NUCLEOTIDE-SEQUENCE; PHOSPHOTRANSFERASE GENE; MULTIPLE ALIGNMENT; TYROSINE KINASE; RESISTANCE; CHLAMYDOMONAS; RECOGNITION; PHOSPHORYLATION; GRANATICOLOR; Streptomyces rimosus; heterologous expression; computer analysis; aminoglycoside 3 '-phosphotransferase; eukaryotic protein kinase; functional sites |
| Subjects: | 500 Science > 570 Life sciences |
| Divisions: | Biology, Preclinical Medicine > Institut für Biophysik und physikalische Biochemie |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 17 Nov 2021 15:58 |
| Last Modified: | 17 Nov 2021 15:58 |
| URI: | https://pred.uni-regensburg.de/id/eprint/40794 |
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