Hutterer, R. and Hof, Martin (2002) Probing ethanol-induced phospholipid phase transitions by the polarity sensitive fluorescence probes Prodan and Patman. ZEITSCHRIFT FUR PHYSIKALISCHE CHEMIE-INTERNATIONAL JOURNAL OF RESEARCH IN PHYSICAL CHEMISTRY & CHEMICAL PHYSICS, 216. pp. 333-346. ISSN 0942-9352
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The emission behaviour of the two polarity sensitive probes Prodan and Patman in phospholipid vesicles was studied as a function of the concentration of ethanol. Comparing the spectral shifts in both the symmetric lipid 1.2-dipalmitoyl-sn-glycero-3-phosphatidylcholine (DPPC) showing a phase transition from a normal to a fully interdigitated gel phase and the strongly asymmetric lipid 1-stearoyl-2-lauroyl-sn-glycero-3-phosphatidylcholine (C(18):C(12)-PC) favouring a mixed interdigitated gel phase A e show that the huge red shifts of Prodan in presence of higher ethanol concentrations cannot be easily attributed to a specific lipid phase transition. Rather, probe relocation and a pronounced increase in solvent relaxation (SR) as monitored by time-resolved emission spectra (TRES) in presence of ethanol contribute to the large shifts observable in both lipid systems in case of Prodan, While Patman exhibits a red shift caused by increased SR due to the ethanol induced formation of a fully interdigitated phase in DPPC, hardly any shift occurs in C(18):C(12)-PC, which is supposed not to undergo an ethanol-induced phase transition.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | INTERDIGITATED GEL PHASE; CHAIN PHOSPHATIDYLCHOLINE BILAYERS; INDUCED LIPID INTERDIGITATION; ANTHROYLOXY FATTY-ACIDS; SOLVENT RELAXATION; MULTILAMELLAR VESICLES; MEMBRANES; BEHAVIOR; DIPALMITOYLPHOSPHATIDYLCHOLINE; DISPERSIONS; Prodan; Patman; solvent relaxation; time-resolved emission spectra; ethanol-induced phase transition |
| Subjects: | 500 Science > 540 Chemistry & allied sciences |
| Divisions: | Chemistry and Pharmacy > Institut für Analytische Chemie, Chemo- und Biosensorik |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 22 Nov 2021 14:11 |
| Last Modified: | 22 Nov 2021 14:11 |
| URI: | https://pred.uni-regensburg.de/id/eprint/40853 |
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