Vogt, Thomas and McClelland, M. and Jung, B. and Popova, S. and Bogenrieder, T. and Becker, B. and Rumpler, G. and Landthaler, M. and Stolz, W. (2001) Progression and NSAID-induced apoptosis in malignant melanomas are independent of cyclooxygenase II. MELANOMA RESEARCH, 11 (6). pp. 587-599. ISSN 0960-8931, 1473-5636
Full text not available from this repository. (Request a copy)Abstract
Cyclooxygenase-II (Cox-II) overexpression is involved in the progression of various subtypes of cancer. We investigated the significance of Cox-II in the progression of malignant melanomas (MMs). Using immunohistology we determined that Cox-II is riot expressed in 70 benign and malignant melanocytic tumours. Basal cell carcinomas (BCCs) and squamous cell carcinomas (SCCs) were also analysed as controls: the BCCs were consistently Cox-II negative (n = 11), whereas the SCCs showed moderate to strong Cox-II expression in 53% (n = 17). Reverse transcription-polymerase chain reaction and Western blotting of MM cell lines and MM tissues confirmed the lack of Cox-if expression in MM. However, in vitro the Cox-inhibiting nonsteroidal anti-inflammatory drug (NSAID) sulindac sulphide (SIS) was significantly more effective in inducing apoptosis than sulindac sulphone (SOS), a derivative with a negligible effect on Cox (P<0.01). The SIS doses needed for the induction of apoptosis were not significantly different in MM cell lines versus a Cox-II-positive colon carcinoma cell line (HT29). Furthermore, add-back experiments with high doses of the prostaglandins PGE(2) and PGF(2)<alpha>, major Cox-II products, did not abrogate this effect. We conclude that Cox-11 expression is not involved in the progression of MM and NSAID-induced apoptosis in MM cell lines seems to follow pathways independent of Cox-II. Nevertheless, Cox-II inhibitors are still candidates for therapy, though they act via an unknown mechanism. (C) 2001 Lippincott Williams & Wilkins.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | NONSTEROIDAL ANTIINFLAMMATORY DRUGS; CELLS GENERATED INSITU; COLON-CANCER CELLS; INCREASED EXPRESSION; COLORECTAL-CANCER; LUNG METASTASIS; NUDE-MICE; IN-VIVO; INHIBITION; INDOMETHACIN; apoptosis; cyclooxygenase; melanoma; NSAIDs; sulindac |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Dermatologie und Venerologie |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 24 Nov 2021 08:14 |
| Last Modified: | 24 Nov 2021 08:14 |
| URI: | https://pred.uni-regensburg.de/id/eprint/40913 |
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