Kuerner, Jens M. and Klimant, Ingo and Krause, Christian and Preu, Harald and Kunz, Werner and Wolfbeis, Otto S. (2001) Inert phosphorescent nanospheres as markers for optical assays. BIOCONJUGATE CHEMISTRY, 12 (6). pp. 883-889. ISSN 1043-1802
Full text not available from this repository. (Request a copy)Abstract
A simple encapsulation technique is presented to produce highly phosphorescent, inert nanospheres that are suitable luminescent markers. It is based on the coprecipitation of phosphorescent ruthenium(II)-tris(polypyridyl) complexes and polyacrylonitrile, (PAN) derivatives from a solution in IV V-dimethylformamide. The beads precipitate in the form of very small aggregates of spherical shape and a typical particle diameter of less than 50 nm. This process allows the encapsulation of phosphorescent and fluorescent dyes in an individual nanosphere provided that they are sufficiently lipophilic. Quenching by oxygen is negligible due to the use of PAN. The nanospheres were characterized with respect to their spectral properties (quantum yields of the luminophores, brightness, luminescence decay time), stability in aqueous buffered suspensions, and in terms of size, shape, and surface charge of the particles, as well as storage stability, quenching by oxygen, and dye leaching.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | LUMINESCENCE; COMPLEXES; IMMUNOASSAYS; PHOTOCHEMISTRY; PHOTOPHYSICS; SENSORS; |
| Subjects: | 500 Science > 540 Chemistry & allied sciences |
| Divisions: | Chemistry and Pharmacy > Institut für Analytische Chemie, Chemo- und Biosensorik > Chemo- und Biosensorik (Prof. Antje J. Bäumner, formerly Prof. Wolfbeis) Chemistry and Pharmacy > Institut für Physikalische und Theoretische Chemie > Chair of Chemistry VI - Physical Chemistry (Solution Chemistry) > Prof. Dr. Werner Kunz |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 29 Nov 2021 09:48 |
| Last Modified: | 29 Nov 2021 09:48 |
| URI: | https://pred.uni-regensburg.de/id/eprint/40956 |
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