Farkas, Stefan and Herfarth, H. and Rossle, M. and Schroeder, J. and Steinbauer, M. and Guba, M. and Beham, A. and Schoelmerich, Juergen and Jauch, K. W. and Anthuber, M. (2001) Quantification of mucosal leucocyte endothelial cell interaction by in vivo fluorescence microscopy in experimental colitis in mice. CLINICAL AND EXPERIMENTAL IMMUNOLOGY, 126 (2). pp. 250-258. ISSN 0009-9104
Full text not available from this repository. (Request a copy)Abstract
Leucocyte recruitment to sites of intestinal inflammation is a crucial, multi-step process that leads ultimately to the accumulation of cells in the inflamed tissue. We established a new in vivo model system of experimental colitis to quantify leucocyte-endothelial cell interaction and leucocyte extravasation in the inflamed mucosa of the colon. Furthermore, we investigated the pathophysiological role of ICAM-1 in the intestinal microcirculation in vivo. Using the model of dextran sodium sulphate (DSS)-induced acute and chronic colitis in mice, in vivo microscopy was performed in the colonic submucosal postcapillary venules and the submucosal collecting venules in normal or inflamed murine colonic segments. ICAM-1 expression was blocked by an anti-ICAM-1 monoclonal antibody or by suppressing NF-kappaB activation by gliotoxin. Significant increases in leucocyte adhesiveness (51-fold in postcapillary venules, 30-fold in collecting venules, P < 0.01) and extravasation (6.5-fold) could be demonstrated as early as day 2 of DSS-application in acute colitis (P < 0.01). This was paralleled by increases in both the histological damage scores and myeloperoxidase activities. In chronic dextran sodium sulphate-induced colitis significant increases in leucocyte-endothelium interactions and leucocyte extravasation were observed. Blocking ICAM-1 expression with a monoclonal antibody or gliotoxin, leucocyte sticking and extravasation were significantly down-regulated in vivo compared to controls (> 70%; P < 0.01). This new model system offers the possibility to specifically assess the role of adhesion molecules in the colonic mucosa in vivo as well as to investigate and quantify the effectiveness of experimental therapeutic approaches in acute or chronic intestinal inflammation.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | INFLAMMATORY BOWEL-DISEASE; DEXTRAN SULFATE SODIUM; NF-KAPPA-B; ADHESION MOLECULE-1 SICAM-1; ULCERATIVE-COLITIS; INTESTINAL INFLAMMATION; (DSS)-INDUCED COLITIS; INTRAVITAL MICROSCOPY; REPERFUSION INJURY; EXPRESSION; experimental colitis; ICAM-1; in vivo microscopy; NF-kappa B |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Chirurgie Medicine > Lehrstuhl für Innere Medizin I Medicine > Lehrstuhl für Pathologie |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 29 Nov 2021 14:10 |
| Last Modified: | 29 Nov 2021 14:10 |
| URI: | https://pred.uni-regensburg.de/id/eprint/40962 |
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