Brunner, Henri and Hausmann, F. and Krieg, R. C. and Endlicher, E. and Schoelmerich, Juergen and Knuechel, R. and Messmann, H. (2001) The effects of 5-aminolevulinic acid esters on protoporphyrin IX production in human adenocarcinoma cell lines. PHOTOCHEMISTRY AND PHOTOBIOLOGY, 74 (5). pp. 721-725. ISSN 0031-8655
Full text not available from this repository. (Request a copy)Abstract
Photodynamic diagnosis (PDD) and photodynamic therapy (PDT) using 5-aminolevulinic acid (ALA)-induced protoporphyrin IX (PPIX) is an interesting approach to detect and treat dysplasia and early cancers in the gastrointestinal tract. Because of low lipophilicity resulting in poor penetration across cell membranes, high doses of ALA should be administered in order to reach clinically relevant levels of PPIX. One way of increasing PPIX accumulation is derivatization of ALA into a more lipophilic molecule. In our in vitro study, different esterifications of ALA were investigated to analyze the effects on PPIX accumulation in human adenocarcinoma cell lines. For systematic analysis of cell type-specific PPIX accumulation, three human adenocarcinoma cell lines (SW480, HT29 and CaCo2) and a fibroblast cell line (CCD18) were tested. 3-(4,5-Dimethylthiazole-2-yl)-2,5-biphenyl tetrazolium bromide (MTT) assays were performed to ensure that the ALA esters showed no cellular dark toxicity. Different concentrations (ranging from 0.012 to 0.6 mmol/L, 3 h) and incubation times (5, 10, 30, 180 min; 0.12 mmol/L) were examined. PPIX accumulation was measured using flow cytometry. ALA esters, especially ALA-hexylester and ALA-benzylester, induced significant higher PPIX levels in adenocarcinoma cell lines when compared with ALA and may be promising candidates for PDT and PDD.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | PHOTODYNAMIC THERAPY; IN-VITRO; FLUORESCENCE; DERIVATIVES; PRODRUGS; LESIONS; |
| Subjects: | 500 Science > 540 Chemistry & allied sciences 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Innere Medizin I Medicine > Lehrstuhl für Pathologie Chemistry and Pharmacy > Institut für Anorganische Chemie > Alumni or Retired Professors > Prof. Dr. Henri Brunner |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 01 Dec 2021 12:02 |
| Last Modified: | 01 Dec 2021 12:02 |
| URI: | https://pred.uni-regensburg.de/id/eprint/40994 |
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