Boehmer, Martin and Pampaloni, Francesco and Wahl, Michael and Rahn, Hans-Jürgen and Erdmann, Rainer and Enderlein, Joerg (2001) Time-resolved confocal scanning device for ultrasensitive fluorescence detection. REVIEW OF SCIENTIFIC INSTRUMENTS, 72 (11). pp. 4145-4152. ISSN 0034-6748
Full text not available from this repository. (Request a copy)Abstract
A confocal laser-scanning microscope for ultrasensitive fluorescence lifetime imaging on surfaces is presented. The system employs a compact electronics for time-correlated single-photon counting (TCSPC), allowing for measuring fluorescence lifetime with 40 ps time resolution, and for continuously recording photon arrival times with 100 ns time resolution. Additionally developed driver electronics serve for synchronization of scanning and data acquisition, which is significant for achieving high spatial image resolution. The capabilities of the measurement system are demonstrated on imaging single molecules immobilized on glass substrates. Finally, it is shown how the TCSPC capabilities of the system can be used not only for lifetime imaging but also for multichannel measurements. (C) 2001 American Institute of Physics.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | LASER-INDUCED FLUORESCENCE; SINGLE-MOLECULE DETECTION; SPECTROSCOPY; IDENTIFICATION; DYNAMICS; |
| Subjects: | 500 Science > 540 Chemistry & allied sciences |
| Divisions: | Chemistry and Pharmacy > Institut für Analytische Chemie, Chemo- und Biosensorik Chemistry and Pharmacy > Institut für Physikalische und Theoretische Chemie |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 01 Dec 2021 14:13 |
| Last Modified: | 01 Dec 2021 14:13 |
| URI: | https://pred.uni-regensburg.de/id/eprint/41006 |
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