Kuerner, Jens M. and Klimant, Ingo and Krause, Christian and Pringsheim, Erika and Wolfbeis, Otto S. (2001) A new type of phosphorescent nanospheres for use in advanced time-resolved multiplexed bioassays. ANALYTICAL BIOCHEMISTRY, 297 (1). pp. 32-41. ISSN 0003-2697
Full text not available from this repository. (Request a copy)Abstract
A new concept to design phosphorescent nanospheres is presented. The spheres are distinguishable by their individual decay time and spectral distribution of their emission spectra. They are composed of a phosphorescent ruthenium metal-ligand complex (MLC dissolved, along with certain strongly fluorescent cyanine dyes, in modified polyacrylonitrile-based nanospheres. Since the emission spectrum of the MIC overlaps the absorption spectrum of the cyanine and both the NMC (the donor) and the cyanine (the acceptor) are in close spatial proximity, efficient resonance energy transfer (RET) does occur. Thus, the nanospheres emit dual luminescence, one from the acceptor dye and the other from the donor MLC. Variation of the concentrations of the acceptor dye results in a varying efficiency of RET, thus making the spheres distinguishable. Hence, a set of multiplexable sphere labels is obtained by using one MLC (acting as the phosphorescent donor and present in constant concentration) and one acceptor dye (which varies in terms of both spectral properties and concentration). The nanospheres can be identified by the emission maximum (reflecting the kind of acceptor dye) and by decay time (reflecting its concentration). Since the same donor MLC is used throughout, all nanospheres can be excited with the same light source. (C) 2001 Academic Press.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | COMPLEXES; SENSORS; multiplexing luminescence labels; phosphorescence; nanospheres; resonance energy transfer (RET); luminescence decay time |
| Subjects: | 500 Science > 540 Chemistry & allied sciences |
| Divisions: | Chemistry and Pharmacy > Institut für Analytische Chemie, Chemo- und Biosensorik > Chemo- und Biosensorik (Prof. Antje J. Bäumner, formerly Prof. Wolfbeis) |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 06 Dec 2021 07:38 |
| Last Modified: | 06 Dec 2021 07:38 |
| URI: | https://pred.uni-regensburg.de/id/eprint/41046 |
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