Niller, Hans Helmut and Salamon, D. and Takacs, M. and Uhlig, J. and Wolf, H. and Minarovits, J. (2001) Protein-DNA interaction and CpG methylation at rep*/vIL-10p of latent Epstein-Barr virus genomes in lymphoid cell lines. BIOLOGICAL CHEMISTRY, 382 (10). pp. 1411-1419. ISSN 1431-6730
Full text not available from this repository. (Request a copy)Abstract
The viral interleukin-10 promoter (vIL-10p), overlapping the rep* element in the Epstein-Barr virus (EBV) genome, is a promoter element active mostly in the late phase of the lytic cycle and immediately upon infection of B cells. rep* was, through transfection experiments with small plasmids, characterised as a cis element supporting oriP replicative function. In this study, in vivo protein binding and CpG methylation at rep*/vIL-10p were analysed in five cell lines that harbour strictly latent EBV genomes. Contrary to the invariably unmethylated dyad symmetry element (DS) of oriP, rep*/vIL-10p was highly methylated and showed only traces of protein binding in all examined cell lines. This result is in agreement with vIL-10p being an inactive promoter of EBV genomes, and makes it less likely that rep* functions as a replicative-element of latent EBV genomes.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | LIGATION-MEDIATED PCR; MEMBRANE-PROTEIN; STABLE REPLICATION; NUCLEAR ANTIGEN-2; MAMMALIAN-CELLS; LYTIC CYCLE; E2F SITE; EXPRESSION; PROMOTER; ORIGIN; bisulfite sequencing; dimethyl sulfate; genomic footprints; latency; replication |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Medizinische Mikrobiologie und Hygiene |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 06 Dec 2021 09:37 |
| Last Modified: | 06 Dec 2021 09:37 |
| URI: | https://pred.uni-regensburg.de/id/eprint/41053 |
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