Ruther, Joachim and Hagstrom, Asa K. and Brandstetter, Birgit and Hofferberth, John and Bruckmann, Astrid and Semmelmann, Florian and Fink, Michaela and Lowack, Helena and Laberer, Sabine and Niehuis, Oliver and Deutzmann, Rainer and Lofstedt, Christer and Sterner, Reinhard (2016) Epimerisation of chiral hydroxylactones by short-chain dehydrogenases/reductases accounts for sex pheromone evolution in Nasonia. SCIENTIFIC REPORTS, 6: 34697. ISSN 2045-2322,
Full text not available from this repository. (Request a copy)Abstract
Males of all species of the parasitic wasp genus Nasonia use (4R,5S)-5-hydroxy-4-decanolide (RS) as component of their sex pheromone while only N. vitripennis (Nv), employs additionally (4R,5R)-5-hydroxy-4-decanolide (RR). Three genes coding for the NAD(+)-dependent short-chain dehydrogenases/reductases (SDRs) NV10127, NV10128, and NV10129 are linked to the ability of Nv to produce RR. Here we show by assaying recombinant enzymes that SDRs from both Nv and N. giraulti (Ng), the latter a species with only RS in the pheromone, epimerise RS into RR and vice versa with (4R)-5-oxo-4-decanolide as an intermediate. Nv-derived SDR orthologues generally had higher epimerisation rates, which were also influenced by NAD(+) availability. Semiquantitative protein analyses of the pheromone glands by tandem mass spectrometry revealed that NV10127 as well as NV10128 and/or NV10129 were more abundant in Nv compared to Ng. We conclude that the interplay of differential expression patterns and SDR epimerisation rates on the ancestral pheromone component RS accounts for the evolution of a novel pheromone phenotype in Nv.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | DROSOPHILA-MELANOGASTER; FUNCTIONAL ASSIGNMENTS; COURTSHIP BEHAVIOR; CRITICAL RESIDUES; DESATURASE GENE; VITRIPENNIS; MOTHS; BIOSYNTHESIS; PTEROMALIDAE; SPECIFICITY; |
| Subjects: | 500 Science > 570 Life sciences 500 Science > 590 Zoological sciences |
| Divisions: | Biology, Preclinical Medicine > Institut für Zoologie > Chemische Ökologie (Prof. Dr. Joachim Ruther) Biology, Preclinical Medicine > Institut für Biophysik und physikalische Biochemie > Prof. Dr. Reinhard Sterner Biology, Preclinical Medicine > Institut für Biochemie, Genetik und Mikrobiologie > Lehrstuhl für Biochemie I > Prof. Dr. Rainer Deutzmann |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 24 Apr 2019 10:55 |
| Last Modified: | 24 Apr 2019 10:55 |
| URI: | https://pred.uni-regensburg.de/id/eprint/4111 |
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