Specific nucleoprotein complexes within adenovirus capsids

Dobner, Thomas and Buechner, Dagmar and Zeller, Thomas and Wolf, Hans and Niller, Hans Helmut (2001) Specific nucleoprotein complexes within adenovirus capsids. BIOLOGICAL CHEMISTRY, 382 (9). pp. 1373-1377. ISSN 1431-6730

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Abstract

Adenoviral DNA was examined within capsids by dimethyl sulfate footprinting. Protein-DNA interactions were visualized through ligation-mediated PCR (LMPCR). Signals for protein binding were found adjacent to both inverted terminal repeats (ITR). There were no indications of close protein binding at several other loci of the viral genome. Therefore, adenovirus type 5 seems to contain sequence- or locus-specific DNA binding proteins within the virion.

Item Type: Article
Uncontrolled Keywords: LIGATION-MEDIATED PCR; FUNCTIONALLY REDUNDANT; PROTEIN-VII; DNA; TYPE-5; PROMOTER; INVIVO; GENE; ENCAPSIDATION; SEQUENCES; adenovirus type 5; dimethyl sulfate; in viro footprint; ligation-mediated PCR; packaging
Subjects: 600 Technology > 610 Medical sciences Medicine
Divisions: Medicine > Lehrstuhl für Medizinische Mikrobiologie und Hygiene
Depositing User: Dr. Gernot Deinzer
Date Deposited: 13 Dec 2021 05:34
Last Modified: 13 Dec 2021 05:34
URI: https://pred.uni-regensburg.de/id/eprint/41114

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