Hutterer, Rudi and Hof, M. (2001) Dynamics in diether lipid bilayers and interdigitated bilayer structures studied by time-resolved emission spectra, decay time and anisotropy profiles. JOURNAL OF FLUORESCENCE, 11 (3). pp. 227-236. ISSN 1053-0509
Full text not available from this repository. (Request a copy)Abstract
We present a comparative fluorescence spectroscopic investigation of diacyl and diether phosphatidylcholine vesicles using different probes with well-defined localization within either the hydrophilic headgroup region or the hydrophobic part of the bilayer. Time-resolved emission spectra have been used to characterize the solvent relaxation behavior in both symmetric and asymmetric diether and diacyl phosphatidylcholines. It is shown that time-resolved emission spectra of Prodan (6-propionyl-2-(dimethylamino)-naphthalene) and its long-alkyl chain derivative Patman (6-palmitoyl-2-[[trimethylammoniumethyl]methylamino]-naphthalene chloride) are a sensitive tool for the detection of differences in the micropolarities and viscosities at the hydrophobic/hydrophilic membrane interface of diether and diacyl lipids, respectively. Moreover, a new approach for the detection of interdigitated bilayers is discussed. It relies on the construction of anisotropy and decay time profiles for the set of n-anthroyloxy fatty acids and is compared with an older fluorescence assay based on intensity measurements only. The shape of plots of the fluorescence steady-state anisotropy versus the position of the chromophore (anthracene-9-carboxylic acid) combined with fluorescence lifetime measurements can be used to differentiate among non-fully, and mixed interdigitated gel phase structures and to predict structures for new lipid species.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | SURROGATE HAMILTONIAN DESCRIPTION; FEMTOSECOND SOLVATION DYNAMICS; ACYL CHAIN INTERDIGITATION; ANTHROYLOXY FATTY-ACIDS; PHOSPHATIDYLCHOLINE BILAYERS; GEL PHASE; SOLVENT RELAXATION; PHOSPHOLIPID-MEMBRANES; HYDROSTATIC-PRESSURE; MODEL MEMBRANES; ether lipids; lipid interdigitation; solvent relaxation; time-resolved emission spectra; Prodan; Patman; n-anthroyloxy fatty acids |
| Subjects: | 500 Science > 540 Chemistry & allied sciences |
| Divisions: | Chemistry and Pharmacy > Institut für Analytische Chemie, Chemo- und Biosensorik |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 13 Dec 2021 08:07 |
| Last Modified: | 13 Dec 2021 08:07 |
| URI: | https://pred.uni-regensburg.de/id/eprint/41155 |
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