Lechner, Sandra and Mueller-Ladner, Ulf and Neumann, Elena and Dietmaier, Wolfgang and Welsh, John and Schoelmerich, Juergen and Rueschoff, Josef and Kullmann, Frank (2001) Use of simplified transcriptors for the analysis of gene expression profiles in laser-microdissected cell populations. LABORATORY INVESTIGATION, 81 (9). pp. 1233-1242. ISSN 0023-6837
Full text not available from this repository. (Request a copy)Abstract
Because colorectal epithelia are prone to malignant transformation, it is important to understand their normal regulation and then to identify differences between the normal cells and the transformed cells. We investigated the gene expression pattern along colonic crypts using a novel gene expression analysis strategy. We combined laser-mediated microdissection of distinct areas within colonic crypts and used modified RNA arbitrarily primed PCR to generate probes for cDNA array hybridization. In the basal part of the crypt, proliferation-related and cell cycle-related genes such as the multifunctional transcription factor e2f-1 or the mismatch-related gene p58/HHR23B were predominantly expressed. In the lumenal part of the crypt, up-regulations of the cysteine protease mch4 and the proto-oncogene c-jun were found. Our findings indicate that e2f1, p58/HHR23B, and mch4 may be involved in key mechanisms leading to malignant transformation in the colonic crypt. Our results also suggest that the technique elucidated here allows identification of gene expression patterns in distinct areas of intestinal tissue samples.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | ARBITRARILY PRIMED PCR; CAPTURE MICRODISSECTION; SUBTRACTIVE HYBRIDIZATION; MOLECULAR ANALYSIS; CARCINOMA-CELLS; SINGLE CELLS; C-JUN; RNA; TISSUE; IDENTIFICATION; |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Innere Medizin I Medicine > Lehrstuhl für Pathologie |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 13 Dec 2021 09:08 |
| Last Modified: | 13 Dec 2021 09:08 |
| URI: | https://pred.uni-regensburg.de/id/eprint/41159 |
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