Schulz, U. and Munker, R. and Ertl, B. and Holler, Ernst and Kolb, Hans-Jochem (2001) Different types of human leukemias express the message for TNF-alpha and interleukin-10. EUROPEAN JOURNAL OF MEDICAL RESEARCH, 6 (8). pp. 359-363. ISSN 0949-2321
Full text not available from this repository.Abstract
Leukemic cells proliferate under the influence of cytokines. in particular, the colony-stimulating factors stimulate the growth and proliferation of myeloid leukemia cells. Under normal conditions, tumor necrosis factor-alpha (TNF-alpha) is produced by activated monocytes, whereas interleukin- 10 (IL-10) is produced by several cell types like monocytes and lymphocytes. The autocrine production of cytokines by leukemic cells may have a pathogenic role in the progression of leukemia or may be an epiphenomenon. In this study, we investigated the expression of TNF-alpha and IL-10 in human leukemic cells by RT-PCR and by a cytoplasmic protein assay. Most cases of acute myelogenous leukemia (AML) (7/8 cases) and all cases of acute lymphoblastic leukemia (ALL) (n = 2), chronic myelogenous leukemia (CML) (n = 5), both in chronic phase and during blast crisis, expressed the mRNA for TNF-alpha and IL-10. A patient whose blasts were positive for IL-10 and negative for TNF-alpha, had high circulating levels of IL-10 and failed to respond to donor lymphocyte infusions. Using a cytoplasmic protein assay, generally low levels of cytoplasmic TNF-alpha and IL-10 were found which increased in case of TNF-alpha following stimulation with lipopolysaccharide. Taken together, our data show that most leukemic cells express the mRNA for a proinflammatory cytokine (TNF-alpha) and an immunosuppressive cytokine (IL-10). More work is necessary to determine under which conditions these cytokines actually paralyze the immune system and thereby permit the progression of leukemia.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | COLONY-STIMULATING FACTOR; INHIBITS CYTOKINE PRODUCTION; HUMAN DENDRITIC CELLS; CLASS-II; ANTIGEN PRESENTATION; DOWN-REGULATION; IL-10; PROLIFERATION; MOLECULES; MONOCYTES; leukemia; TNF-alpha; IL-10 |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Innere Medizin III (Hämatologie und Internistische Onkologie) |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 14 Dec 2021 05:35 |
| Last Modified: | 14 Dec 2021 05:35 |
| URI: | https://pred.uni-regensburg.de/id/eprint/41185 |
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