Laszlo, V. and Rothe, G. and Hegyesi, H. and Szeberenyi, J. B. and Orso, E. and Schmitz, Gerd and Falus, A. (2001) Increased histidine decarboxylase expression during in vitro monocyte maturation; a possible role of endogenously synthesised histamine in monocyte/macrophage differentiation. INFLAMMATION RESEARCH, 50 (8). pp. 428-434. ISSN 1023-3830, 1420-908X
Full text not available from this repository. (Request a copy)Abstract
Objective: In this study the expression of histidine decarboxylase (HDC), the pivotal enzyme in histamine formation and the effect of endogenously produced histamine on differentiation antigens was examined during in vitro differentiation of human monocytes. Material and treatment., Human elutriated monocytes from healthy volunteers were incubated with macrophage colony stimulating factor (M-CSF) and the expression of HDC was followed at both mRNA and protein levels. To study the possible function of histamine we followed the expression of some cell surface markers (CD14, CD16, CD91, CD49d and CD11c) relevant for phagocytic differentiation upon incubation in the presence of different histamine inhibitors, an HDC inhibitor: S(+)-alpha -fluoromethyl-histidine HCl, (alpha FMH), a compound that disturbs the interaction of histamine with intracellular cyp450 moieties: N,N-diethyl-2-[4-(phenyl-methyl) phenoxy]-ethanamine HCl. (DPPE); and H-1 and H-2 receptor antagonists, Triprolidine and Cimetidine. Results: During in vitro culture of elutriated human monocytes, in the presence of M-CSF, the gene expression and biosynthesis of HDC was considerably increased. The various antihistamine agents decreased th expression of the cell surface markers examined in this study. Conclusions:These data support the elevation of HDC expression during human monocytic differentiation and the possibility that monocyte-derived histamine is partially involved in regulation of M-CSF induced in vitro human monocyte/macrophage phagocytic differentiation.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | COLONY-STIMULATING FACTOR; INTRACELLULAR HISTAMINE; FLOW-CYTOMETRY; T-CELLS; MACROPHAGES; INTERLEUKIN-3; ANTAGONIST; RECEPTORS; CYTOKINES; MESSENGER; monocyte; histamine; differentiation; M-CSF |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Klinische Chemie und Laboratoriumsmedizin |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 14 Dec 2021 09:49 |
| Last Modified: | 14 Dec 2021 09:49 |
| URI: | https://pred.uni-regensburg.de/id/eprint/41223 |
Actions (login required)
 |
View Item |
