Dolzer, Jan and Krannich, S. and Fischer, K. and Stengl, Monika (2001) Oscillations of the transepithelial potential of moth olfactory sensilla are influenced by octopamine and serotonin. JOURNAL OF EXPERIMENTAL BIOLOGY, 204 (16). pp. 2781-2794. ISSN 0022-0949
Full text not available from this repository. (Request a copy)Abstract
(T)he biogenic amine octopamine is known to enhance the sensitivity of male moths to their species-specific pheromones in flight-tunnel experiments. This sensitization of pheromone-guided upwind flight is at least partly due to octopamine-dependent increases in the peak nerve impulse frequency of the pheromone response of olfactory receptor neurons. It is not known, however, whether octopamine exerts its effects directly on the electrical properties of the olfactory receptor neurons or indirectly, via modulation of the accessory cells of the sensillum. In extracellular tip recordings of pheromone-dependent trichoid sensilla on the antennae of male Manduca sexta moths, we investigated the effects of octopamine and serotonin on the transepithelial potential, which is generated by the activity of V-ATPases in sensillar accessory cells. In addition, the action potential activity of unstimulated olfactory receptor neurons was examined in the presence of biogenic amines. Under constant environmental conditions, the transepithelial potential oscillated regularly with periods of 2-8min and with a 1-25mV peak-to-peak amplitude over periods of several hours. These oscillatory intervals were interrupted by periods of relatively stable transepithelial potential, correlated with flight activity by the moth. Octopamine reduced the amplitude of the transepithelial potential oscillation and decreased the resistance of the sensillum preparation in a dose-dependent manner. Serotonin altered the waveform of the transepithelial potential, but did not change the resistance of the preparation. Thus, both amines affect the accessory cells, but have different targets in the regulation of the transepithelial potential. Neither amine significantly influenced the spontaneous action potential activity of the olfactory receptor neurons.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | SECONDARY ACTIVE-TRANSPORT; INSECT PLASMA-MEMBRANE; CABBAGE-LOOPER MOTH; MANDUCA-SEXTA; PHEROMONE-RESPONSE; MODULATORY ACTION; RECEPTOR NEURONS; MALPIGHIAN TUBULES; PHOTOPERIOD CUES; TRICHOPLUSIA-NI; Manduca sexta; pheromone sensillum; tip recording; transepithelial potential; oscillation; action potential; octopamine; serotonin |
| Subjects: | 500 Science > 590 Zoological sciences |
| Divisions: | Biology, Preclinical Medicine > Institut für Zoologie > Zoologie/Evolutionsbiologie (Prof. Dr. Jürgen Heinze) |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 14 Dec 2021 10:25 |
| Last Modified: | 14 Dec 2021 10:25 |
| URI: | https://pred.uni-regensburg.de/id/eprint/41228 |
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