Oswald, Bernhard and Gruber, Michaela and Boehmer, Martin and Lehman, Frank and Probst, Mario and Wolfbeis, Otto S. (2001) Novel diode laser-compatible fluorophores and their application to single molecule detection, protein labeling and fluorescence resonance energy transfer immunoassay. PHOTOCHEMISTRY AND PHOTOBIOLOGY, 74 (2). pp. 237-245. ISSN 0031-8655, 1751-1097
Full text not available from this repository. (Request a copy)Abstract
We describe a series of new long-wave absorbing and fluorescing cyanine dyes and labels (based on a general logic for the design of such dyes), their spectra, covalent and noncovalent linkage to proteins, their use in single molecule detection (SMD) and as donors and acceptors, respectively, in fluorescence resonance energy transfer studies. The new labels represent water-soluble and reactive fluorophores whose quantum yields increase substantially if noncovalently or covalently bound to proteins. Due to their strong absorptions between 550 and 700 nm they are excitable by light-emitting diodes or diode lasers. Their high absorbances (epsilon around 100000) and adequate fluorescence quantum yields (phi up to 0.68 if bound to proteins) along with their availability as reactive NHS esters make them viable labels for proteins and oligomers, e.g. in context with SMD or fluorescence energy transfer immunoassay which is demonstrated for the system HSA/anti-HSA.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | DYES; REAGENTS; |
| Subjects: | 500 Science > 540 Chemistry & allied sciences |
| Divisions: | Chemistry and Pharmacy > Institut für Analytische Chemie, Chemo- und Biosensorik > Chemo- und Biosensorik (Prof. Antje J. Bäumner, formerly Prof. Wolfbeis) |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 17 Jan 2022 08:11 |
| Last Modified: | 17 Jan 2022 08:11 |
| URI: | https://pred.uni-regensburg.de/id/eprint/41238 |
Actions (login required)
 |
View Item |
